将ATF2 ORF插入酵母磷酸甘油酸激酶I基因PGK1的BglII酶切位点，构建质粒pUC-PIA2K。以IAH基因作为同源臂，以Kan基因作为酵母转化子选择的标记。
参考文献：Zhang J, Zhang C, Qi Y, Dai L, Ma H, Guo X, Xiao D. Acetate ester production by Chinese yellow rice wine yeast overexpressing the alcohol acetyltransferase-encoding gene ATF2. Genet Mol Res. 2014 Nov 27;13(4):9735-46.
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中国黄酒酵母过表达酒精乙酰转移酶编码基因ATF2生产醋酸酯
将ATF2 ORF插入酵母磷酸甘油酸激酶I基因PGK1的BglII酶切位点，构建质粒pUC-PIA2K。以IAH基因作为同源臂，以Kan基因作为酵母转化子选择的标记。
参考文献：Zhang J, Zhang C, Qi Y, Dai L, Ma H, Guo X, Xiao D. Acetate ester production by Chinese yellow rice wine yeast overexpressing the alcohol acetyltransferase-encoding gene ATF2. Genet Mol Res. 2014 Nov 27;13(4):9735-46.
