产品名称: | Recombinant Saccharomyces cerevisiae Mitochondrial FAD-linked sulfhydryl oxidase ERV1 (ERV1) |
货号: | TSANTI000410 |
纯度: | Greater than 85% as determined by SDS-PAGE. |
基因名: | ERV1 |
Uniprot No.: | P27882 |
别名: | (14 kDa regulatory protein)(Essential for respiration and vegetative growth protein 1) |
种属: | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) |
蛋白长度: | Full Length of Mature Protein |
来源: | Yeast |
分子量: | 22.5 kDa |
表达区域: | 1-189aa |
氨基酸序列 | MKAIDKMTDNPPQEGLSGRKIIYDEDGKPCRSCNTLLDFQYVTGKISNGLKNLSSNGKLAGTGALTGEASELMPGSRTYRKVDPPDVEQLGRSSWTLLHSVAASYPAQPTDQQKGEMKQFLNIFSHIYPCNWCAKDFEKYIRENAPQVESREELGRWMCEAHNKVNKKLRKPKFDCNFWEKRWKDGWDE |
蛋白标签: | C-terminal 6xHis-tagged |
产品提供形式: | Liquid or Lyophilized powder |
缓冲液: | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
复溶: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
储存条件: | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
保质期: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
货期: | Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
注意事项: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Datasheet & COA: | Please contact us to get it. |
功能: | FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins. |
基因功能参考文献: | Chimeras between the N-terminal arm of ScErv1 and a variety of truncated LtErv constructs were able to rescue yeast cells that lack ScErv1. PMID: 29310075 |
亚细胞定位: | Mitochondrion intermembrane space. |
数据库链接: | KEGG: sce:YGR029W |
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产品名称: | Recombinant Saccharomyces cerevisiae Mitochondrial FAD-linked sulfhydryl oxidase ERV1 (ERV1) |
货号: | TSANTI000410 |
纯度: | Greater than 85% as determined by SDS-PAGE. |
基因名: | ERV1 |
Uniprot No.: | P27882 |
别名: | (14 kDa regulatory protein)(Essential for respiration and vegetative growth protein 1) |
种属: | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) |
蛋白长度: | Full Length of Mature Protein |
来源: | Yeast |
分子量: | 22.5 kDa |
表达区域: | 1-189aa |
氨基酸序列 | MKAIDKMTDNPPQEGLSGRKIIYDEDGKPCRSCNTLLDFQYVTGKISNGLKNLSSNGKLAGTGALTGEASELMPGSRTYRKVDPPDVEQLGRSSWTLLHSVAASYPAQPTDQQKGEMKQFLNIFSHIYPCNWCAKDFEKYIRENAPQVESREELGRWMCEAHNKVNKKLRKPKFDCNFWEKRWKDGWDE |
蛋白标签: | C-terminal 6xHis-tagged |
产品提供形式: | Liquid or Lyophilized powder |
缓冲液: | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
复溶: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
储存条件: | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
保质期: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
货期: | Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
注意事项: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Datasheet & COA: | Please contact us to get it. |
功能: | FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins. |
基因功能参考文献: | Chimeras between the N-terminal arm of ScErv1 and a variety of truncated LtErv constructs were able to rescue yeast cells that lack ScErv1. PMID: 29310075 |
亚细胞定位: | Mitochondrion intermembrane space. |
数据库链接: | KEGG: sce:YGR029W |