Recombinant Saccharomyces cerevisiae NADPH-dependent methylglyoxal reductase GRE2 (GRE2)
Recombinant Saccharomyces cerevisiae NADPH-dependent methylglyoxal reductase GRE2 (GRE2)
规格:
货期:
编号:TSANTI000419
品牌:Testobio
| 产品名称: | Recombinant Saccharomyces cerevisiae NADPH-dependent methylglyoxal reductase GRE2 (GRE2) |
| 货号: | TSANTI000419 |
| 纯度： | Greater than 90% as determined by SDS-PAGE. |
| 基因名： | GRE2 |
| Uniprot No.： | Q12068 |
| 种属： | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) |
| 蛋白长度： | Full Length |
| 来源： | E.coli |
| 分子量： | 44.2 kDa |
| 表达区域： | 1-342aa |
| 氨基酸序列 | MSVFVSGANGFIAQHIVDLLLKEDYKVIGSARSQEKAENLTEAFGNNPKFSMEVVPDISKLDAFDHVFQKHGKDIKIVLHTASPFCFDITDSERDLLIPAVNGVKGILHSIKKYAADSVERVVLTSSYAAVFDMAKENDKSLTFNEESWNPATWESCQSDPVNAYCGSKKFAEKAAWEFLEENRDSVKFELTAVNPVYVFGPQMFDKDVKKHLNTSCELVNSLMHLSPEDKIPELFGGYIDVRDVAKAHLVAFQKRETIGQRLIVSEARFTMQDVLDILNEDFPVLKGNIPVGKPGSGATHNTLGATLDNKKSKKLLGFKFRNLKETIDDTASQILKFEGRI |
| 蛋白标签： | N-terminal 10xHis-tagged |
| 产品提供形式： | Liquid or Lyophilized powder |
| 缓冲液： | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| 复溶： | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| 储存条件： | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| 保质期： | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
| 货期： | 3-7 business days |
| 注意事项： | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA： | Please contact us to get it. |
| 功能： | Catalyzes the irreversible reduction of the cytotoxic compound methylglyoxal (MG, 2-oxopropanal) to (S)-lactaldehyde as an alternative to detoxification of MG by glyoxalase I GLO1. MG is synthesized via a bypath of glycolysis from dihydroxyacetone phosphate and is believed to play a role in cell cycle regulation and stress adaptation. Also catalyzes the reduction of 3-methylbutanal to 3-methylbutanol. Acts as a suppressor of 3-methylbutanol-induced filamentation by modulating the levels of 3-methylbutanal, the signal to which cells respond by filamentation. Also involved in ergosterol metabolism. |
| 基因功能参考文献： | Gre2 forms a homodimer, each subunit of which contains an N-terminal Rossmann-fold domain and a variable C-terminal domain, which participates in substrate recognition. PMID: 24879127 |
| 亚细胞定位： | Cytoplasm. Nucleus. |
| 蛋白家族： | NAD(P)-dependent epimerase/dehydratase family, Dihydroflavonol-4-reductase subfamily |
| 数据库链接： | KEGG: sce:YOL151W |
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Recombinant Saccharomyces cerevisiae NADPH-dependent methylglyoxal reductase GRE2 (GRE2)
Recombinant Saccharomyces cerevisiae NADPH-dependent methylglyoxal reductase GRE2 (GRE2)
规格:
货期:
编号:TSANTI000419
品牌:Testobio
| 产品名称: | Recombinant Saccharomyces cerevisiae NADPH-dependent methylglyoxal reductase GRE2 (GRE2) |
| 货号: | TSANTI000419 |
| 纯度： | Greater than 90% as determined by SDS-PAGE. |
| 基因名： | GRE2 |
| Uniprot No.： | Q12068 |
| 种属： | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) |
| 蛋白长度： | Full Length |
| 来源： | E.coli |
| 分子量： | 44.2 kDa |
| 表达区域： | 1-342aa |
| 氨基酸序列 | MSVFVSGANGFIAQHIVDLLLKEDYKVIGSARSQEKAENLTEAFGNNPKFSMEVVPDISKLDAFDHVFQKHGKDIKIVLHTASPFCFDITDSERDLLIPAVNGVKGILHSIKKYAADSVERVVLTSSYAAVFDMAKENDKSLTFNEESWNPATWESCQSDPVNAYCGSKKFAEKAAWEFLEENRDSVKFELTAVNPVYVFGPQMFDKDVKKHLNTSCELVNSLMHLSPEDKIPELFGGYIDVRDVAKAHLVAFQKRETIGQRLIVSEARFTMQDVLDILNEDFPVLKGNIPVGKPGSGATHNTLGATLDNKKSKKLLGFKFRNLKETIDDTASQILKFEGRI |
| 蛋白标签： | N-terminal 10xHis-tagged |
| 产品提供形式： | Liquid or Lyophilized powder |
| 缓冲液： | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| 复溶： | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| 储存条件： | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| 保质期： | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
| 货期： | 3-7 business days |
| 注意事项： | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA： | Please contact us to get it. |
| 功能： | Catalyzes the irreversible reduction of the cytotoxic compound methylglyoxal (MG, 2-oxopropanal) to (S)-lactaldehyde as an alternative to detoxification of MG by glyoxalase I GLO1. MG is synthesized via a bypath of glycolysis from dihydroxyacetone phosphate and is believed to play a role in cell cycle regulation and stress adaptation. Also catalyzes the reduction of 3-methylbutanal to 3-methylbutanol. Acts as a suppressor of 3-methylbutanol-induced filamentation by modulating the levels of 3-methylbutanal, the signal to which cells respond by filamentation. Also involved in ergosterol metabolism. |
| 基因功能参考文献： | Gre2 forms a homodimer, each subunit of which contains an N-terminal Rossmann-fold domain and a variable C-terminal domain, which participates in substrate recognition. PMID: 24879127 |
| 亚细胞定位： | Cytoplasm. Nucleus. |
| 蛋白家族： | NAD(P)-dependent epimerase/dehydratase family, Dihydroflavonol-4-reductase subfamily |
| 数据库链接： | KEGG: sce:YOL151W |