产品名称: | Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply) |
货号: | TSANTI000434 |
纯度: | Greater than 90% as determined by SDS-PAGE. |
基因名: | ply |
Uniprot No.: | P0C2J9 |
种属: | Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) |
蛋白长度: | Full Length of Mature Protein |
来源: | E.coli |
分子量: | 52.8 kDa |
表达区域: | 2-471aa |
氨基酸序列 | ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND |
蛋白标签: | Tag-Free |
产品提供形式: | Liquid or Lyophilized powder |
缓冲液: | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
复溶: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
储存条件: | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
保质期: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
货期: | 3-7 business days |
注意事项: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Datasheet & COA: | Please contact us to get it. |
功能: | A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation. |
亚细胞定位: | Secreted. Secreted, cell wall. Host cell membrane; Multi-pass membrane protein. |
蛋白家族: | Thiol-activated cytolysin family |
数据库链接: | KEGG: spn:SP_1923 |
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产品名称: | Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply) |
货号: | TSANTI000434 |
纯度: | Greater than 90% as determined by SDS-PAGE. |
基因名: | ply |
Uniprot No.: | P0C2J9 |
种属: | Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) |
蛋白长度: | Full Length of Mature Protein |
来源: | E.coli |
分子量: | 52.8 kDa |
表达区域: | 2-471aa |
氨基酸序列 | ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND |
蛋白标签: | Tag-Free |
产品提供形式: | Liquid or Lyophilized powder |
缓冲液: | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
复溶: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
储存条件: | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
保质期: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
货期: | 3-7 business days |
注意事项: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Datasheet & COA: | Please contact us to get it. |
功能: | A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation. |
亚细胞定位: | Secreted. Secreted, cell wall. Host cell membrane; Multi-pass membrane protein. |
蛋白家族: | Thiol-activated cytolysin family |
数据库链接: | KEGG: spn:SP_1923 |