Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial
Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial
规格:
货期:
编号:TSANTI000455
品牌:Testobio
| 产品名称: | Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial |
| 货号: | TSANTI000455 |
| 纯度： | Greater than 85% as determined by SDS-PAGE. |
| 生物活性： | Not Test |
| 基因名： | RAP1 |
| Uniprot No.： | P11938 |
| 别名： | (Repressor/activator site-binding protein)(SBF-E)(TUF) |
| 种属： | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) |
| 蛋白长度： | Partial |
| 来源： | E.coli |
| 分子量： | 28.7 |
| 表达区域： | 353-598aa |
| 氨基酸序列 | GALPSHNKASFTDEEDEFILDVVRKNPTRRTTHTLYDEISHYVPNHTGNSIRHRFRVYLSKRLEYVYEVDKFGKLVRDDDGNLIKTKVLPPSIKRKFSADEDYTLAIAVKKQFYRDLFQIDPDTGRSLITDEDTPTAIARRNMTMDPNHVPGSEPNFAAYRTQSRRGPIAREFFKHFAEEHAAHTENAWRDRFRKFLLAYGIDDYISYYEAEKAQNREPEPMKNLTNRPKRPGVPTPGNYNSAAKR |
| 蛋白标签： | Tag-Free |
| 产品提供形式： | Liquid or Lyophilized powder |
| 缓冲液： | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| 复溶： | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| 储存条件： | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| 保质期： | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
| 货期： | 3-7 business days |
| 注意事项： | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| Datasheet & COA： | Please contact us to get it. |
| 功能： | Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C(1-3)A] sequences. The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers. RAP1 associates with SIR3 and SIR4 proteins to form a DNA-binding complex that initiates the repression at the HM loci and telomeres. May also target the binding of RIF1 and RIF2 to silencers and telomeres. Forms with GCR1 a transcriptional activation complex that is required for expression of glycolytic and ribosomal gene. |
| 基因功能参考文献： | Rap1 (ScRap1) can either activate or repress transcription. This bimodal transcriptional activity has led to the widespread study of the mode of action of ScRap1. This review summarizes current knowledge about ScRap1, including its structure, mechanisms of transcription regulation, and biological functions, and the future directions in the field. Review. PMID: 27100480 |
| 亚细胞定位： | Nucleus. Chromosome, telomere. |
| 蛋白家族： | RAP1 family |
| 数据库链接： | KEGG: sce:YNL216W |
首页/产品中心/蛋白
产品中心
联系我们 CONTACT US
0574-87917803
testobio@163.com
浙江省宁波市镇海区庄市街道兴庄路9号
创e慧谷42号楼B幢401室
最新促销
Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial
Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial
规格:
货期:
编号:TSANTI000455
品牌:Testobio
| 产品名称: | Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial |
| 货号: | TSANTI000455 |
| 纯度： | Greater than 85% as determined by SDS-PAGE. |
| 生物活性： | Not Test |
| 基因名： | RAP1 |
| Uniprot No.： | P11938 |
| 别名： | (Repressor/activator site-binding protein)(SBF-E)(TUF) |
| 种属： | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) |
| 蛋白长度： | Partial |
| 来源： | E.coli |
| 分子量： | 28.7 |
| 表达区域： | 353-598aa |
| 氨基酸序列 | GALPSHNKASFTDEEDEFILDVVRKNPTRRTTHTLYDEISHYVPNHTGNSIRHRFRVYLSKRLEYVYEVDKFGKLVRDDDGNLIKTKVLPPSIKRKFSADEDYTLAIAVKKQFYRDLFQIDPDTGRSLITDEDTPTAIARRNMTMDPNHVPGSEPNFAAYRTQSRRGPIAREFFKHFAEEHAAHTENAWRDRFRKFLLAYGIDDYISYYEAEKAQNREPEPMKNLTNRPKRPGVPTPGNYNSAAKR |
| 蛋白标签： | Tag-Free |
| 产品提供形式： | Liquid or Lyophilized powder |
| 缓冲液： | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| 复溶： | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| 储存条件： | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| 保质期： | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
| 货期： | 3-7 business days |
| 注意事项： | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| Datasheet & COA： | Please contact us to get it. |
| 功能： | Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C(1-3)A] sequences. The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers. RAP1 associates with SIR3 and SIR4 proteins to form a DNA-binding complex that initiates the repression at the HM loci and telomeres. May also target the binding of RIF1 and RIF2 to silencers and telomeres. Forms with GCR1 a transcriptional activation complex that is required for expression of glycolytic and ribosomal gene. |
| 基因功能参考文献： | Rap1 (ScRap1) can either activate or repress transcription. This bimodal transcriptional activity has led to the widespread study of the mode of action of ScRap1. This review summarizes current knowledge about ScRap1, including its structure, mechanisms of transcription regulation, and biological functions, and the future directions in the field. Review. PMID: 27100480 |
| 亚细胞定位： | Nucleus. Chromosome, telomere. |
| 蛋白家族： | RAP1 family |
| 数据库链接： | KEGG: sce:YNL216W |