Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA)
Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA)
规格:
货期:
编号:TSANTI000461
品牌:Testobio
| 产品名称: | Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA) |
| 货号: | TSANTI000461 |
| 纯度： | Greater than 85% as determined by SDS-PAGE. |
| 生物活性： | Not Test |
| 基因名： | ptxA |
| Uniprot No.： | P04977 |
| 别名： | (PTX S1)(Islet-activating protein S1)(IAP S1)(NAD-dependent ADP-ribosyltransferase) |
| 种属： | Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) 百日咳博德特菌 |
| 蛋白长度： | Full Length of Mature Protein |
| 来源： | E.coli |
| 分子量： | 40.8 |
| 表达区域： | 35-269aa |
| 氨基酸序列 | DDPPATVYRYDSRPPEDVFQNGFTAWGNNDNVLDHLTGRSCQVGSSNSAFVSTSSSRRYTEVYLEHRMQEAVEAERAGRGTGHFIGYIYEVRADNNFYGAASSYFEYVDTYGDNAGRILAGALATYQSEYLAHRRIPPENIRRVTRVYHNGITGETTTTEYSNARYVSQQTRANPNPYTSRRSVASIVGTLVRMAPVIGACMARQAESSEAMAAWSERAGEAMVLVYYESIAYSF |
| 蛋白标签： | N-terminal 6xHis-B2M-tagged |
| 产品提供形式： | Liquid or Lyophilized powder |
| 缓冲液： | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| 复溶： | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| 储存条件： | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| 保质期： | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
| 货期： | 3-7 business days |
| 注意事项： | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| Datasheet & COA： | Please contact us to get it. |
| 功能： | S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors. |
| 基因功能参考文献： | It has been proposed that it is the systemic activity of pertussis toxin and not pulmonary pathology that promotes mortality in critical pertussis. PMID: 28784932 |
| 亚细胞定位： | Secreted. Note=The individual chains are secreted by a sec-dependent mechanism into the periplasm. Then, S1 associates with the outer membrane before it joins with the B subunit to form the secretion-competent holotoxin. The type IV secretion system ptl mediates secretion of assembled toxin through the outer membrane. |
| 蛋白家族： | Bacterial exotoxin subunit A family |
| 数据库链接： | KEGG: bpe:BP3783 |
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Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA)
Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA)
规格:
货期:
编号:TSANTI000461
品牌:Testobio
| 产品名称: | Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA) |
| 货号: | TSANTI000461 |
| 纯度： | Greater than 85% as determined by SDS-PAGE. |
| 生物活性： | Not Test |
| 基因名： | ptxA |
| Uniprot No.： | P04977 |
| 别名： | (PTX S1)(Islet-activating protein S1)(IAP S1)(NAD-dependent ADP-ribosyltransferase) |
| 种属： | Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) 百日咳博德特菌 |
| 蛋白长度： | Full Length of Mature Protein |
| 来源： | E.coli |
| 分子量： | 40.8 |
| 表达区域： | 35-269aa |
| 氨基酸序列 | DDPPATVYRYDSRPPEDVFQNGFTAWGNNDNVLDHLTGRSCQVGSSNSAFVSTSSSRRYTEVYLEHRMQEAVEAERAGRGTGHFIGYIYEVRADNNFYGAASSYFEYVDTYGDNAGRILAGALATYQSEYLAHRRIPPENIRRVTRVYHNGITGETTTTEYSNARYVSQQTRANPNPYTSRRSVASIVGTLVRMAPVIGACMARQAESSEAMAAWSERAGEAMVLVYYESIAYSF |
| 蛋白标签： | N-terminal 6xHis-B2M-tagged |
| 产品提供形式： | Liquid or Lyophilized powder |
| 缓冲液： | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| 复溶： | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| 储存条件： | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| 保质期： | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
| 货期： | 3-7 business days |
| 注意事项： | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| Datasheet & COA： | Please contact us to get it. |
| 功能： | S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors. |
| 基因功能参考文献： | It has been proposed that it is the systemic activity of pertussis toxin and not pulmonary pathology that promotes mortality in critical pertussis. PMID: 28784932 |
| 亚细胞定位： | Secreted. Note=The individual chains are secreted by a sec-dependent mechanism into the periplasm. Then, S1 associates with the outer membrane before it joins with the B subunit to form the secretion-competent holotoxin. The type IV secretion system ptl mediates secretion of assembled toxin through the outer membrane. |
| 蛋白家族： | Bacterial exotoxin subunit A family |
| 数据库链接： | KEGG: bpe:BP3783 |