产品名称: | Recombinant Bacteroides fragilis Chaperone protein htpG (htpG), partial |
货号: | TSANTI000512 |
纯度: | Greater than 85% as determined by SDS-PAGE. |
基因名: | htpG |
Uniprot No.: | Q5LCH4 |
别名: | htpG; BF2491; Chaperone protein HtpG; Heat shock protein HtpG; High temperature protein G |
种属: | Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) |
蛋白长度: | Partial |
来源: | E.coli |
分子量: | 57.0 kDa |
表达区域: | 1-326aa |
氨基酸序列 | MQKGNIGVTTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLNTLASISEFKGELGDLTVHVSLGKDTITISDRGIGLTAEEIDKYINQIAFSGANDFLEKYKNDANAIIGHFGLGFYSAFMVSKKVEIITKSYKEGAQAVKWTCDGSPEFTLEEVEKADRGTDIVLYIDDDCKEFLEESRISALLKKYCSFLPVPIAFGKKKEWKDGKQVETAEDNVINDTIPLWTKKPSELSDEDYKKFYRELYPMSDEPLFWIHLNVDYPFHLTGILYFPKVKSNIDLNKNKIQLYCNQVYVTDSVEGIVPDFLTLLHGVLDSPDIPLNVSR |
蛋白标签: | N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
产品提供形式: | Liquid or Lyophilized powder |
缓冲液: | Tris-based buffer,50% glycerol |
储存条件: | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
保质期: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
货期: | 3-7 business days |
注意事项: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
产品描述: | Recombinant B. fragilis htpG expression in E.coli requires the insertion of the target DNA fragment into an E.coli expression vector, routinely a plasmid vector, and the transferral of this vector into E.coli cells. The cells are then cultured and induced to express this htpG protein. The cells are harvested by centrifugation, samples prepared and proteins detected by polyacrylamide gel electrophoresis and subsequent staining of the gel with Coomassie Brilliant Blue or silver stain or by immunoblotting. The htpG protein expression in E.coli is highly scalable and can be adjusted from the bacterial colony to conical flasks for liquid cultures, to fermentation reaction chambers. |
Datasheet & COA: | Please contact us to get it. |
功能: | Molecular chaperone. Has ATPase activity. |
亚细胞定位: | Cytoplasm. |
蛋白家族: | Heat shock protein 90 family |
数据库链接: | KEGG: bfs:BF9343_2410 |
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产品名称: | Recombinant Bacteroides fragilis Chaperone protein htpG (htpG), partial |
货号: | TSANTI000512 |
纯度: | Greater than 85% as determined by SDS-PAGE. |
基因名: | htpG |
Uniprot No.: | Q5LCH4 |
别名: | htpG; BF2491; Chaperone protein HtpG; Heat shock protein HtpG; High temperature protein G |
种属: | Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) |
蛋白长度: | Partial |
来源: | E.coli |
分子量: | 57.0 kDa |
表达区域: | 1-326aa |
氨基酸序列 | MQKGNIGVTTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLNTLASISEFKGELGDLTVHVSLGKDTITISDRGIGLTAEEIDKYINQIAFSGANDFLEKYKNDANAIIGHFGLGFYSAFMVSKKVEIITKSYKEGAQAVKWTCDGSPEFTLEEVEKADRGTDIVLYIDDDCKEFLEESRISALLKKYCSFLPVPIAFGKKKEWKDGKQVETAEDNVINDTIPLWTKKPSELSDEDYKKFYRELYPMSDEPLFWIHLNVDYPFHLTGILYFPKVKSNIDLNKNKIQLYCNQVYVTDSVEGIVPDFLTLLHGVLDSPDIPLNVSR |
蛋白标签: | N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
产品提供形式: | Liquid or Lyophilized powder |
缓冲液: | Tris-based buffer,50% glycerol |
储存条件: | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
保质期: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
货期: | 3-7 business days |
注意事项: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
产品描述: | Recombinant B. fragilis htpG expression in E.coli requires the insertion of the target DNA fragment into an E.coli expression vector, routinely a plasmid vector, and the transferral of this vector into E.coli cells. The cells are then cultured and induced to express this htpG protein. The cells are harvested by centrifugation, samples prepared and proteins detected by polyacrylamide gel electrophoresis and subsequent staining of the gel with Coomassie Brilliant Blue or silver stain or by immunoblotting. The htpG protein expression in E.coli is highly scalable and can be adjusted from the bacterial colony to conical flasks for liquid cultures, to fermentation reaction chambers. |
Datasheet & COA: | Please contact us to get it. |
功能: | Molecular chaperone. Has ATPase activity. |
亚细胞定位: | Cytoplasm. |
蛋白家族: | Heat shock protein 90 family |
数据库链接: | KEGG: bfs:BF9343_2410 |