产品名称: | Recombinant Helicobacter pylori Urease subunit alpha (ureA) |
货号: | TSANTI000636 |
纯度: | Greater than 85% as determined by SDS-PAGE. |
基因名: | ureA |
Uniprot No.: | P14916 |
别名: | ureA; hpuA; HP_0073; Urease subunit alpha; EC 3.5.1.5; Urea amidohydrolase subunit alpha |
种属: | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
蛋白长度: | Full Length |
来源: | E.coli |
分子量: | 30.5 kDa |
表达区域: | 1-238aa |
氨基酸序列 | MKLTPKELDKLMLHYAGELAKKRKEKGIKLNYVEAVALISAHIMEEARAGKKTAAELMQEGRTLLKPDDVMDGVASMIHEVGIEAMFPDGTKLVTVHTPIEANGKLVPGELFLKNEDITINEGKKAVSVKVKNVGDRPVQIGSHFHFFEVNRCLDFDREKTFGKRLDIASGTAVRFEPGEEKSVELIDIGGNRRIFGFNALVDRQADNESKKIALHRAKERGFHGAKSDDNYVKTIKE |
蛋白标签: | N-terminal 6xHis-tagged |
产品提供形式: | Liquid or Lyophilized powder |
缓冲液: | Tris-based buffer,50% glycerol |
储存条件: | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
保质期: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
货期: | 3-7 business days |
注意事项: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
产品描述: | The synthesis of Recombinant H.pylori ureA protein began with the isolation of ureA gene from the native genome. The next step was to amplify the ureA gene by inserting the ureA gene into an expression vector and then introducing the vector into the selected host cell, E.coli. Significant amounts of recombinant H.pylori ureA protein were produced by the host only when expression genes were added. For the purpose of protein purification, the vector contains N-terminal 6xHis tag to the ureA DNA sequence. The purity of this protein is 85%+ determined by SDS-PAGE. |
Datasheet & COA: | Please contact us to get it. |
功能: | Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach. |
亚细胞定位: | Cytoplasm. Note=Also associates with the outer membrane upon autolysis of neighboring bacteria. |
蛋白家族: | Urease gamma subunit family; Urease beta subunit family |
数据库链接: | KEGG: heo:C694_00355 |
浙江省宁波市镇海区庄市街道兴庄路9号
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产品名称: | Recombinant Helicobacter pylori Urease subunit alpha (ureA) |
货号: | TSANTI000636 |
纯度: | Greater than 85% as determined by SDS-PAGE. |
基因名: | ureA |
Uniprot No.: | P14916 |
别名: | ureA; hpuA; HP_0073; Urease subunit alpha; EC 3.5.1.5; Urea amidohydrolase subunit alpha |
种属: | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
蛋白长度: | Full Length |
来源: | E.coli |
分子量: | 30.5 kDa |
表达区域: | 1-238aa |
氨基酸序列 | MKLTPKELDKLMLHYAGELAKKRKEKGIKLNYVEAVALISAHIMEEARAGKKTAAELMQEGRTLLKPDDVMDGVASMIHEVGIEAMFPDGTKLVTVHTPIEANGKLVPGELFLKNEDITINEGKKAVSVKVKNVGDRPVQIGSHFHFFEVNRCLDFDREKTFGKRLDIASGTAVRFEPGEEKSVELIDIGGNRRIFGFNALVDRQADNESKKIALHRAKERGFHGAKSDDNYVKTIKE |
蛋白标签: | N-terminal 6xHis-tagged |
产品提供形式: | Liquid or Lyophilized powder |
缓冲液: | Tris-based buffer,50% glycerol |
储存条件: | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
保质期: | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
货期: | 3-7 business days |
注意事项: | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
产品描述: | The synthesis of Recombinant H.pylori ureA protein began with the isolation of ureA gene from the native genome. The next step was to amplify the ureA gene by inserting the ureA gene into an expression vector and then introducing the vector into the selected host cell, E.coli. Significant amounts of recombinant H.pylori ureA protein were produced by the host only when expression genes were added. For the purpose of protein purification, the vector contains N-terminal 6xHis tag to the ureA DNA sequence. The purity of this protein is 85%+ determined by SDS-PAGE. |
Datasheet & COA: | Please contact us to get it. |
功能: | Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach. |
亚细胞定位: | Cytoplasm. Note=Also associates with the outer membrane upon autolysis of neighboring bacteria. |
蛋白家族: | Urease gamma subunit family; Urease beta subunit family |
数据库链接: | KEGG: heo:C694_00355 |