从含有该基因的载体pVT100-LDH中用PCR扩增出的干酪乳杆菌LDH，质粒p426-GPD用EcoRI酶切，用碱性磷酸酶去磷酸化，与PCR产物共转化。酿酒酵母JEN1和ADY2从之前克隆的质粒p416-GPD转移到p415-GPD，用BamHI和XhoI酶切将质粒将JEN1和ADY2分离，并与相同酶切的p415- GPD连接。
参考文献：Pacheco A, Talaia G, Sá-Pessoa J, Bessa D, Gonçalves MJ, Moreira R, Paiva S, Casal M, Queirós O. Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2. FEMS Yeast Res. 2012 May;12(3):375-81.
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从含有该基因的载体pVT100-LDH中用PCR扩增出的干酪乳杆菌LDH，质粒p426-GPD用EcoRI酶切，用碱性磷酸酶去磷酸化，与PCR产物共转化。酿酒酵母JEN1和ADY2从之前克隆的质粒p416-GPD转移到p415-GPD，用BamHI和XhoI酶切将质粒将JEN1和ADY2分离，并与相同酶切的p415- GPD连接。
参考文献：Pacheco A, Talaia G, Sá-Pessoa J, Bessa D, Gonçalves MJ, Moreira R, Paiva S, Casal M, Queirós O. Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2. FEMS Yeast Res. 2012 May;12(3):375-81.
