pSa143包含构巢曲菌的整个argB基因,去除含有酵母LEU2和2Jlm DNA的两个小EcoRI片段得到pSa123,向pSa123插入来自pKA5-1包含米曲霉补充蛋氨酸营养缺陷型突变的BamHI片段得到pRBMI。

参考文献:Katsuya Gomi, Yuzuru Iimura & Shodo Hara (1987) Integrative
Transformation of Aspergillus oryzae with a Plasmid Containing the Aspergillus nidulans argB Gene, Agricultural and Biological Chemistry, 51:9, 2549-2555
pSa143包含构巢曲菌的整个argB基因,去除含有酵母LEU2和2Jlm DNA的两个小EcoRI片段得到pSa123,向pSa123插入来自pKA5-1包含米曲霉补充蛋氨酸营养缺陷型突变的BamHI片段得到pRBMI。

参考文献:Katsuya Gomi, Yuzuru Iimura & Shodo Hara (1987) Integrative
Transformation of Aspergillus oryzae with a Plasmid Containing the Aspergillus nidulans argB Gene, Agricultural and Biological Chemistry, 51:9, 2549-2555