HB101
F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-
Please note that different sources have different genotypes so treat this information with caution.
From a GIBCO BRL list of competent cells.
Hybrid of E. coli K12 and E. coli B (but 98% K strain AB266 according to Smith et al.)
Host for pBR322 and many plasmids
Sigma lists the deletion Δ(gpt,proA). Check this.
Promega does not list F-, mcrB, or mrr
Streptomycin resistant
References:
Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.
Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56 - pdf version from Invitrogen
Lacks S and Greenberg JR (1977) J Mol Biol 114:153.
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