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2022-04-26 03:31:26       wshphd

HB101
F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-
Please note that different sources have different genotypes so treat this information with caution.

From a GIBCO BRL list of competent cells.

Hybrid of E. coli K12 and E. coli B (but 98% K strain AB266 according to Smith et al.)

Host for pBR322 and many plasmids

Sigma lists the deletion Δ(gpt,proA). Check this.

Promega does not list F-, mcrB, or mrr

Streptomycin resistant

References:

Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.

Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56 - pdf version from Invitrogen

Lacks S and Greenberg JR (1977) J Mol Biol 114:153.

另外参考:
http://www.takara.com.cn/explain/M/D9021.pdf


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