将KanMX-Cre/loxP重组系统与URA反选择相结合，构建了改进的工具箱pUMRI。将PGAL1、PGAL10、PGAL2和PGAL7这四个GAL启动子引入pUMRI盒，分别生成pUMRI-A和pUMRI-b。
参考文献：Lv X, Wang F, Zhou P, Ye L, Xie W, Xu H, Yu H. Dual regulation of cytoplasmic and mitochondrial acetyl-CoA utilization for improved isoprene production in Saccharomyces cerevisiae. Nat Commun. 2016 Sep 21;7:12851.
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提高酿酒酵母异戊二烯的生产
将KanMX-Cre/loxP重组系统与URA反选择相结合，构建了改进的工具箱pUMRI。将PGAL1、PGAL10、PGAL2和PGAL7这四个GAL启动子引入pUMRI盒，分别生成pUMRI-A和pUMRI-b。
参考文献：Lv X, Wang F, Zhou P, Ye L, Xie W, Xu H, Yu H. Dual regulation of cytoplasmic and mitochondrial acetyl-CoA utilization for improved isoprene production in Saccharomyces cerevisiae. Nat Commun. 2016 Sep 21;7:12851.
