构建了aflR破坏载体pXHSE。pXN1中6.7kb含niad的HindIII-XbaI片段用T4 DNA聚合酶钝化，连接pACH，用SmaI和EcoRV预处理，再次钝端，去磷酸化生成pXHSE。pXHSE中的niad可选择标记取代了aflR编码区。
参考文献：Takahashi T, Chang PK, Matsushima K, Yu J, Abe K, Bhatnagar D, Cleveland TE, Koyama Y. Nonfunctionality of Aspergillus sojae aflR in a strain of Aspergillus parasiticus with a disrupted aflR gene. Appl Environ Microbiol. 2002 Aug;68(8):3737-43.
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构建了aflR破坏载体pXHSE。pXN1中6.7kb含niad的HindIII-XbaI片段用T4 DNA聚合酶钝化，连接pACH，用SmaI和EcoRV预处理，再次钝端，去磷酸化生成pXHSE。pXHSE中的niad可选择标记取代了aflR编码区。
参考文献：Takahashi T, Chang PK, Matsushima K, Yu J, Abe K, Bhatnagar D, Cleveland TE, Koyama Y. Nonfunctionality of Aspergillus sojae aflR in a strain of Aspergillus parasiticus with a disrupted aflR gene. Appl Environ Microbiol. 2002 Aug;68(8):3737-43.
