金黄色葡萄球菌(Staphylococcus aureus，S. aureus)也称“金葡菌”，隶属于葡萄球菌属，是革兰氏阳性菌代表，为一种常见的食源性致病微生物。该菌最适宜生长温度为37℃，pH为7.4，耐高盐，可在盐浓度接近10%的环境中生长。属于厚壁菌门，所以细胞壁特别厚，很难做成感受态，难以转染。很难做成荧光标记的菌株。
Genetic Characteristics：
Staphylococcus aureus Xen36 was derived from the parental strain Staphylococcus aureus ATCC49525 (Wright), a clinical isolate from bacteremia
patient. S. aureus Xen 36 possesses a stable copy of the modified Photorhabdus luminescens luxABCDE operon at a single integration site on a native plasmid.
Growth Characteristics：
S. aureus Xen36 grows well in various mediaincluding Brain Heart Infusion (BHI), TrypticaseSoy Broth (TSB), and Luria Bertani (LB) at 37ºC under ambient aeration. S. aureus Xen36 may also be grown selectively on medium containing 200 μg/ml kanamycin.
发表评论