NCIMB number | NCIMB 14684 |
Deposit type | Bacteria |
Type strain | Yes |
GMO | No |
Taxon name | Oceanobacillus polygoni |
Depositor designation | SA9 |
Preservation method | Lyophilised |
Preserved as | Virgibacillus polygonumi |
Price band | A |
Media | 211 |
Gas regime | aerobic |
Incubation period | 4 days |
Growth factors (and/or information) | Can be grown at 25C or 30C |
ACDP category | 1 |
Colony Edge | Entire |
Colony Surface | Smooth & Shiny |
Colony Shape | Spreadingalongstreak |
Colony Elevation | Flat |
Colony Colour | Off White |
Colony Opacity | Translucent&Opaque |
Cellular Shape | Fat Rods |
Cellular Size | medium |
Cellular Arrangement | Singly |
Gram stain | Gram Positive |
Cellular (Other) | Sides/Ends Parallel/Rounded |
Depositor Name | Isao Yumoto |
Depositor Company | Bioproduction Research Institute, |
Depositor Address | Nat. Inst. Advanced Ind. Sci & Technology. 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku Sapporo 062-8517 Japan |
Source | "Indigo fermentation liquor, Japan" |
Isolated by | Yoshiko Hanaoka |
Date of Isolation | 01/01/2009 |
Date of Accession | 28/06/2010 |
Other collection IDs | JCM17252 |
Yeast? | False |
K12 | No |
PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/23504965 |
浙江省宁波市镇海区庄市街道兴庄路9号
创e慧谷42号楼B幢401室
NCIMB number | NCIMB 14684 |
Deposit type | Bacteria |
Type strain | Yes |
GMO | No |
Taxon name | Oceanobacillus polygoni |
Depositor designation | SA9 |
Preservation method | Lyophilised |
Preserved as | Virgibacillus polygonumi |
Price band | A |
Media | 211 |
Gas regime | aerobic |
Incubation period | 4 days |
Growth factors (and/or information) | Can be grown at 25C or 30C |
ACDP category | 1 |
Colony Edge | Entire |
Colony Surface | Smooth & Shiny |
Colony Shape | Spreadingalongstreak |
Colony Elevation | Flat |
Colony Colour | Off White |
Colony Opacity | Translucent&Opaque |
Cellular Shape | Fat Rods |
Cellular Size | medium |
Cellular Arrangement | Singly |
Gram stain | Gram Positive |
Cellular (Other) | Sides/Ends Parallel/Rounded |
Depositor Name | Isao Yumoto |
Depositor Company | Bioproduction Research Institute, |
Depositor Address | Nat. Inst. Advanced Ind. Sci & Technology. 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku Sapporo 062-8517 Japan |
Source | "Indigo fermentation liquor, Japan" |
Isolated by | Yoshiko Hanaoka |
Date of Isolation | 01/01/2009 |
Date of Accession | 28/06/2010 |
Other collection IDs | JCM17252 |
Yeast? | False |
K12 | No |
PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/23504965 |
使用说明: | 获得菌株后,原则上应尽早进行活化和保存。若不能立即活化,应按照说明书放入相应的环境中进行保存。 若菌株为厌氧菌,请按厌氧菌培养要求在厌氧环境中操作。 (1)若保存的菌株为冻干粉形式,可加入少量生理盐水或非选择性肉汤培养基进行溶解悬浮, 用划线或涂布方式接种至平板,或着吸取菌液至液体培养基中进行培养。 (2)若保存的菌株为甘油冻存或液体保存形式,可在室温速融后划线或涂布接种至平板,或直接加入到液体培养基中进行培养。 (3)若保存的菌株为斜面或半固体形式,可直接挑取菌苔转接至平板或液体培养基中进行培养。 活化菌株使用的培养基的成分通常包括以下几个组分: (a)基本营养成分,如蛋白胨、牛肉浸粉、酵母浸粉等,主要提供碳源、氮源及微量元素等; (b)氯化钠:维持一定的渗透压环境; (c)磷酸盐:维持pH的相对稳定; (d)特殊成分:如血清、半胱氨酸、氯化血红素等,促进特定菌的生长。 根据菌株的生长特性,选择合适的培养基或对特定的培养基添加成分进行改良,以满足菌株的生长需求 |
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