NCIMB number | NCIMB 14621 |
Deposit type | Bacteria |
Type strain | Yes |
GMO | No |
Taxon name | Methylobacterium nodulans |
Depositor designation | ORS 2060 |
Preservation method | Lyophilised |
Other catalogue information | Induces nitrogen-fixing root nodules on legume Crotalaria spp. |
Price band | A |
Media | 259 |
Gas regime | aerobic |
Incubation period | 4 days |
ACDP category | 1 |
Colony Edge | Entire |
Colony Surface | Smooth & Shiny |
Colony Shape | Circular |
Colony Elevation | Low Convex |
Colony Colour | Off White |
Colony Opacity | Opaque |
Cellular Shape | Short Rods |
Cellular Arrangement | Random/Singly |
Gram stain | Gram Negative |
Cellular (Other) | Sides/Ends Parallel/Rounded |
Depositor Company | BCCM/LMG Bacteria Collection |
Depositor Address | Laboratorium voor Microbiologie, Universiteit Gent (UGent), K.L. Ledeganckstraat 35 B-9000 Gent Belgium |
Source | Legume (Crotalaria podocarpa) Senegal |
Date of Accession | 15/03/2010 |
History | "LMG -- Ghent University, Belgium -- P de Lajudie LSTM IRD Monpellier, France" |
Other collection IDs | CNCM I 2342 LMG21967 |
GenInfo Identifier (EMBL, GenBank & DDBJ) | AF220763 |
Yeast? | False |
K12 | No |
References | JOURAND (P.), GIRAUD (E.), B�NA (G.), SY (A.), WILLEMS (A.), GILLIS (M.), DREYFUS (B.) and DE LAJUDIE (P.): Methylobacterium nodulans sp. nov., for a group of aerobic, facultatively methylotrophic, legume root-nodule-forming and nitrogen-fixing bacteria. Int. J. Syst. Evol. Microbiol., 2004, 54, 2269-2273 |
浙江省宁波市镇海区庄市街道兴庄路9号
创e慧谷42号楼B幢401室
NCIMB number | NCIMB 14621 |
Deposit type | Bacteria |
Type strain | Yes |
GMO | No |
Taxon name | Methylobacterium nodulans |
Depositor designation | ORS 2060 |
Preservation method | Lyophilised |
Other catalogue information | Induces nitrogen-fixing root nodules on legume Crotalaria spp. |
Price band | A |
Media | 259 |
Gas regime | aerobic |
Incubation period | 4 days |
ACDP category | 1 |
Colony Edge | Entire |
Colony Surface | Smooth & Shiny |
Colony Shape | Circular |
Colony Elevation | Low Convex |
Colony Colour | Off White |
Colony Opacity | Opaque |
Cellular Shape | Short Rods |
Cellular Arrangement | Random/Singly |
Gram stain | Gram Negative |
Cellular (Other) | Sides/Ends Parallel/Rounded |
Depositor Company | BCCM/LMG Bacteria Collection |
Depositor Address | Laboratorium voor Microbiologie, Universiteit Gent (UGent), K.L. Ledeganckstraat 35 B-9000 Gent Belgium |
Source | Legume (Crotalaria podocarpa) Senegal |
Date of Accession | 15/03/2010 |
History | "LMG -- Ghent University, Belgium -- P de Lajudie LSTM IRD Monpellier, France" |
Other collection IDs | CNCM I 2342 LMG21967 |
GenInfo Identifier (EMBL, GenBank & DDBJ) | AF220763 |
Yeast? | False |
K12 | No |
References | JOURAND (P.), GIRAUD (E.), B�NA (G.), SY (A.), WILLEMS (A.), GILLIS (M.), DREYFUS (B.) and DE LAJUDIE (P.): Methylobacterium nodulans sp. nov., for a group of aerobic, facultatively methylotrophic, legume root-nodule-forming and nitrogen-fixing bacteria. Int. J. Syst. Evol. Microbiol., 2004, 54, 2269-2273 |
使用说明: | 获得菌株后,原则上应尽早进行活化和保存。若不能立即活化,应按照说明书放入相应的环境中进行保存。 若菌株为厌氧菌,请按厌氧菌培养要求在厌氧环境中操作。 (1)若保存的菌株为冻干粉形式,可加入少量生理盐水或非选择性肉汤培养基进行溶解悬浮, 用划线或涂布方式接种至平板,或着吸取菌液至液体培养基中进行培养。 (2)若保存的菌株为甘油冻存或液体保存形式,可在室温速融后划线或涂布接种至平板,或直接加入到液体培养基中进行培养。 (3)若保存的菌株为斜面或半固体形式,可直接挑取菌苔转接至平板或液体培养基中进行培养。 活化菌株使用的培养基的成分通常包括以下几个组分: (a)基本营养成分,如蛋白胨、牛肉浸粉、酵母浸粉等,主要提供碳源、氮源及微量元素等; (b)氯化钠:维持一定的渗透压环境; (c)磷酸盐:维持pH的相对稳定; (d)特殊成分:如血清、半胱氨酸、氯化血红素等,促进特定菌的生长。 根据菌株的生长特性,选择合适的培养基或对特定的培养基添加成分进行改良,以满足菌株的生长需求 |
---|