NCIMB number | NCIMB 14760 |
Deposit type | Bacteria |
Type strain | No |
GMO | No |
Taxon name | Gluconacetobacter tumulicola |
Depositor designation | K5929-2-1b |
Preservation method | Unknown |
Price band | A |
Media | 354 |
Gas regime | aerobic |
Incubation period | 2 days |
ACDP category | 1 |
Colony Edge | Entire |
Colony Surface | Smooth And Shiny |
Colony Shape | Circular |
Colony Elevation | Low Convex |
Colony Colour | Beige |
Colony Opacity | Translucent |
Cellular Shape | Rods |
Cellular Size | short |
Cellular Arrangement | Singles And Groups |
Gram stain | Gram Negative |
Depositor Name | Junta Sugiyama |
Depositor Company | TechnoSuruga Laboratory Co.,Ltd |
Depositor Address | Chiba Branch Office & Lab, 2102 -10 Dainichi, Yotsukaido-shi Chiba-ken Japan 284-0001 |
Source | "Black viscous substance in a plaster hole on central area of the ceiling in the stone chamber of the Kitora Tumulus is Asuka village, Nara Pref. Japan" |
Isolated by | Nozomi Tazato |
Date of Isolation | 09/12/2005 |
Date of Accession | 06/06/2011 |
Yeast? | False |
K12 | No |
References | Gluconacetobacter tumulicola sp. nov. and Gluconacetobacter asukensis sp. nov., isolated from the stone chamber interior of the Kitora Tumulus, Int J Syst Evol Microbiol 62(8), 2032-2038, 2012 |
PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/22038998 |
浙江省宁波市镇海区庄市街道兴庄路9号
创e慧谷42号楼B幢401室
NCIMB number | NCIMB 14760 |
Deposit type | Bacteria |
Type strain | No |
GMO | No |
Taxon name | Gluconacetobacter tumulicola |
Depositor designation | K5929-2-1b |
Preservation method | Unknown |
Price band | A |
Media | 354 |
Gas regime | aerobic |
Incubation period | 2 days |
ACDP category | 1 |
Colony Edge | Entire |
Colony Surface | Smooth And Shiny |
Colony Shape | Circular |
Colony Elevation | Low Convex |
Colony Colour | Beige |
Colony Opacity | Translucent |
Cellular Shape | Rods |
Cellular Size | short |
Cellular Arrangement | Singles And Groups |
Gram stain | Gram Negative |
Depositor Name | Junta Sugiyama |
Depositor Company | TechnoSuruga Laboratory Co.,Ltd |
Depositor Address | Chiba Branch Office & Lab, 2102 -10 Dainichi, Yotsukaido-shi Chiba-ken Japan 284-0001 |
Source | "Black viscous substance in a plaster hole on central area of the ceiling in the stone chamber of the Kitora Tumulus is Asuka village, Nara Pref. Japan" |
Isolated by | Nozomi Tazato |
Date of Isolation | 09/12/2005 |
Date of Accession | 06/06/2011 |
Yeast? | False |
K12 | No |
References | Gluconacetobacter tumulicola sp. nov. and Gluconacetobacter asukensis sp. nov., isolated from the stone chamber interior of the Kitora Tumulus, Int J Syst Evol Microbiol 62(8), 2032-2038, 2012 |
PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/22038998 |
使用说明: | 获得菌株后,原则上应尽早进行活化和保存。若不能立即活化,应按照说明书放入相应的环境中进行保存。 若菌株为厌氧菌,请按厌氧菌培养要求在厌氧环境中操作。 (1)若保存的菌株为冻干粉形式,可加入少量生理盐水或非选择性肉汤培养基进行溶解悬浮, 用划线或涂布方式接种至平板,或着吸取菌液至液体培养基中进行培养。 (2)若保存的菌株为甘油冻存或液体保存形式,可在室温速融后划线或涂布接种至平板,或直接加入到液体培养基中进行培养。 (3)若保存的菌株为斜面或半固体形式,可直接挑取菌苔转接至平板或液体培养基中进行培养。 活化菌株使用的培养基的成分通常包括以下几个组分: (a)基本营养成分,如蛋白胨、牛肉浸粉、酵母浸粉等,主要提供碳源、氮源及微量元素等; (b)氯化钠:维持一定的渗透压环境; (c)磷酸盐:维持pH的相对稳定; (d)特殊成分:如血清、半胱氨酸、氯化血红素等,促进特定菌的生长。 根据菌株的生长特性,选择合适的培养基或对特定的培养基添加成分进行改良,以满足菌株的生长需求 |
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