NCIMB number | NCIMB 15067 |
Deposit type | Bacteria |
Taxon name | Mycobacterium sp. |
Depositor designation | 40; SN 1900 |
Preservation method | Liquid nitrogen & Lyophilised |
Media | 119 |
Gas regime | Aerobic |
ACDP category | 1 |
Colony Edge | Entire |
Colony Surface | Smooth and rough |
Colony Elevation | Papillate |
Colony Colour | Orange |
Colony Opacity | Opaque |
Cellular Shape | Rods |
Cellular Size | Small |
Cellular Arrangement | Grouped |
Gram stain | Variable |
Depositor Name | Imen Nouioui |
Depositor Company | Newcastle University |
Depositor Address | School of Biology, Devonshire Building, 5th floor Newcastle University, Newcastle upon Tyne NE1 7RU, UK |
Isolated by | J. Grange |
Date of Accession | 14/11/2016 |
浙江省宁波市镇海区庄市街道兴庄路9号
创e慧谷42号楼B幢401室
NCIMB number | NCIMB 15067 |
Deposit type | Bacteria |
Taxon name | Mycobacterium sp. |
Depositor designation | 40; SN 1900 |
Preservation method | Liquid nitrogen & Lyophilised |
Media | 119 |
Gas regime | Aerobic |
ACDP category | 1 |
Colony Edge | Entire |
Colony Surface | Smooth and rough |
Colony Elevation | Papillate |
Colony Colour | Orange |
Colony Opacity | Opaque |
Cellular Shape | Rods |
Cellular Size | Small |
Cellular Arrangement | Grouped |
Gram stain | Variable |
Depositor Name | Imen Nouioui |
Depositor Company | Newcastle University |
Depositor Address | School of Biology, Devonshire Building, 5th floor Newcastle University, Newcastle upon Tyne NE1 7RU, UK |
Isolated by | J. Grange |
Date of Accession | 14/11/2016 |
使用说明: | 获得菌株后,原则上应尽早进行活化和保存。若不能立即活化,应按照说明书放入相应的环境中进行保存。 若菌株为厌氧菌,请按厌氧菌培养要求在厌氧环境中操作。 (1)若保存的菌株为冻干粉形式,可加入少量生理盐水或非选择性肉汤培养基进行溶解悬浮, 用划线或涂布方式接种至平板,或着吸取菌液至液体培养基中进行培养。 (2)若保存的菌株为甘油冻存或液体保存形式,可在室温速融后划线或涂布接种至平板,或直接加入到液体培养基中进行培养。 (3)若保存的菌株为斜面或半固体形式,可直接挑取菌苔转接至平板或液体培养基中进行培养。 活化菌株使用的培养基的成分通常包括以下几个组分: (a)基本营养成分,如蛋白胨、牛肉浸粉、酵母浸粉等,主要提供碳源、氮源及微量元素等; (b)氯化钠:维持一定的渗透压环境; (c)磷酸盐:维持pH的相对稳定; (d)特殊成分:如血清、半胱氨酸、氯化血红素等,促进特定菌的生长。 根据菌株的生长特性,选择合适的培养基或对特定的培养基添加成分进行改良,以满足菌株的生长需求 |
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