A basic tool kit for the construction of gene expression systems and gene circuits in the yeast Saccharomyces cerevisiae.

This tool kit contains (1) four destination vectors: pDEST-12y (SB-1000®), pDEST-23y (SB-1001®), pDEST-34y (SB-1002®), and pDEST-45y (SB-1003®); (2) three yeast vectors: pCarrier 3 (416) (SB-1004®), pCarrier 7 (426) (SB-1005®), and pCarrier 8 (integ) (SB-1024®); (3) one yeast selection maker: pENTR_L1L2_KanMX (SB-1006®); (4) four yeast constitutive promoters: pENTR_L4R1_pHOG1 (SB-1008®), pENTR_L4R1_pHOT1 (SB-1035®), pENTR_L4R1_pYPD1 (SB-1041®), and pENTR_L4R1_pSKN7 (SB-1105®), (5) one yeast inducible promoter: pENTR_L4R1_pGAL1 (SB-1046®); and (6) two empty Entry vectors: pENTR_L1L2_DONR1 (SB-1013®) and pENTR_L4R1_DONR2 (SB-1014®). Detail information of these plasmids can be found in these products’ information and in the ATCC® Synthetic Biology Solutions User Guide.

• pDEST-12y (ATCC SB-1000)

  pDEST-23y (ATCC SB-1001)

  pDEST-34y (ATCC SB-1002)

  pDEST-45y (ATCC SB-1003)

  
  

  Yeast vectors:

  pCarrier 3 (416) (ATCC SB-1004)

  pCarrier 7 (426) (ATCC SB-1005)

  pCarrier 8 (integ) (ATCC SB-1024)

  
  

  Yeast selection maker:

  pENTR_L1L2_KanMX (ATCC SB-1006)

  
  

  Yeast constitutive promoters:

  pENTR_L4R1_pHOG1 (ATCC SB-1008)

  pENTR_L4R1_pHOT1 (ATCC SB-1035)

  pENTR_L4R1_pYPD1 (ATCC SB-1041)

  pENTR_L4R1_pSKN7 (ATCC SB-1105)

  
  

  Yeast inducible promoter:

  pENTR_L4R1_pGAL1 (ATCC SB-1046)

  
  

  Entry vectors:

  pENTR_L1L2_DONR1 (ATCC SB-1013)

  pENTR_L4R1_DONR2 (ATCC SB-1014)