为了获得整合质粒pBS1C-spaBTCSIFEGRK,我们从该基因的基因组DNA中扩增出整个spa位点spaBTCSIFEGRK,后连接到载体pBS1C与EcoRI和EpeI生成pBS1C-spaBTCSIFEGRK。

参考文献:Zhang Q, Kobras CM, Gebhard S, Mascher T, Wolf D. Regulation of heterologous subtilin production in Bacillus subtilis W168. Microb Cell Fact. 2022 Apr 7;21(1):57.
为了获得整合质粒pBS1C-spaBTCSIFEGRK,我们从该基因的基因组DNA中扩增出整个spa位点spaBTCSIFEGRK,后连接到载体pBS1C与EcoRI和EpeI生成pBS1C-spaBTCSIFEGRK。

参考文献:Zhang Q, Kobras CM, Gebhard S, Mascher T, Wolf D. Regulation of heterologous subtilin production in Bacillus subtilis W168. Microb Cell Fact. 2022 Apr 7;21(1):57.