通过敲除ptsG和pykFA基因，构建了大肠杆菌W3110GFA。与野生型菌株相比，W3110GFA厌氧发酵24 h后，琥珀酸形成增加约3.4倍。
参考文献：Lee SJ, Lee DY, Kim TY, Kim BH, Lee J, Lee SY. Metabolic engineering of Escherichia coli for enhanced production of succinic acid, based on genome comparison and in silico gene knockout simulation. Appl Environ Microbiol. 2005 Dec;71(12):7880-7.
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大肠杆菌提高琥珀酸产量的代谢工程
通过敲除ptsG和pykFA基因，构建了大肠杆菌W3110GFA。与野生型菌株相比，W3110GFA厌氧发酵24 h后，琥珀酸形成增加约3.4倍。
参考文献：Lee SJ, Lee DY, Kim TY, Kim BH, Lee J, Lee SY. Metabolic engineering of Escherichia coli for enhanced production of succinic acid, based on genome comparison and in silico gene knockout simulation. Appl Environ Microbiol. 2005 Dec;71(12):7880-7.
