• PURPOSE

  low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

• Vector backbone

  pSC101

   (Search Vector Database)

• Backbone size w/o insert (bp)9262

• Modifications to backbone

  cloned PybaJ-timerbac in pUA66 with a pSC101 replicon

• Vector type

  Bacterial Expression

GROWTH IN BACTERIA

• Bacterial Resistance(s)

  Kanamycin, 50 μg/mL

• Growth Temperature

  37°C

• Growth Strain(s)

  DH5alpha

• Copy number

  Low Copy

GENE/INSERT

• Gene/Insert name

  TIMER-bac

• Species

  Synthetic; Discosoma spp

• Mutation

  mutations: S4T and S179T

• Promoterconstitutive ybaJp promoter

CLONING INFORMATION

• Cloning methodRestriction Enzyme

• 5′ cloning siteunknown (unknown if destroyed)

• 3′ cloning siteunknown (unknown if destroyed)

• 5′ sequencing primerunknown