1.Streak F. nucleatum ATCC 23726 from a frozen stock on a TSPC agar plate and incubate 3 days in an anaerobic chamber at 37 °C to obtain single colonies.2.Inoculate a colony of F. nucleatum into an 8 mL of TSPC and incubate statically overnight at 37 °C 5% CO2 incubator. The next day, tr...