| 产品名称 | pTYB1 | ||||||||||||||||||
| 商品货号 | TSPLA104052 | ||||||||||||||||||
| 基本信息 |
质粒名称:pTYB1 质粒类型:原核表达载体、大肠杆菌表达载体 载体大小:7477 bp 5 测序引物:T7 载体标签:Intein 载体抗性:Ampicillin (氨苄青霉素) 备注:This C-terminal fusion vector is designed for the in-frame insertion of a target gene into a polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein. |
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| 使用说明 | |||||||||||||||||||
| 图谱 |
| 产品名称 | pTYB1 | ||||||||||||||||||
| 商品货号 | TSPLA104052 | ||||||||||||||||||
| 使用说明 | |||||||||||||||||||
| 图谱 | |||||||||||||||||||
| 详情 |
质粒名称:pTYB1 质粒类型:原核表达载体、大肠杆菌表达载体 载体大小:7477 bp 5 测序引物:T7 载体标签:Intein 载体抗性:Ampicillin (氨苄青霉素) 备注:This C-terminal fusion vector is designed for the in-frame insertion of a target gene into a polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein. |