| 产品名称 | VCSM13辅助噬菌体 | ||||||||||||||||||
| 商品货号 | TSPLA104469 | ||||||||||||||||||
| 基本信息 |
产品名称 VCSM13辅助噬菌体 产品用途 噬菌体表面展示系统辅助噬菌体 产品特性 产生用于测序和突变的单链噬菌粒DNA 产品简介 VCSM13是M13KO7的衍生物，最初由Stratagene商业化，常用于噬菌体展示。VCSM13的表型尚未发表，确切序列尚不清楚。为了繁殖和扩增VCSM13辅助噬菌体，需要使用TG1、ER2738或菌株XL1 Blue MRF′大肠杆菌菌株，因为这些菌株含有supE44突变，该突变提供谷氨酰胺抑制剂tRNA。VCSM13辅助噬菌体严格用于从已切除的噬菌体中产生单链DNA（这也是M13KO7辅助噬菌体的用途）。 规格 1ml/管，1管 贮存 Recommends storing them at -80℃ with 50% Glycerol. As long as there is a -80℃ stock in the lab, the amplified lab prep can be stored at +4℃. 扩增 1. Streak out TG1 onto an 2YT plate. 2. Pick a single colony of TG1 and inoculate into 5ml 2×YT and grow until OD600 = 0.3 (~2.5×108 cells/ml). 3. Add helper phage at a multiplicity of infection (MOI) of 20:1 (phage-to-cells ratio). Note: If amplifying VCSM13 helper phage, add Kanamycin to a final concentration of 25μg/ml to the medium 30 minutes after the helper phage and cells have been allowed to grow together. 4. Grow the culture at 37℃ with vigorous aeration (~300rpm) for 8 hours. 5. Heat the culture to 65℃ for 15 minutes. 6. Spin down the cell debris and transfer the supernatant to a fresh tube. 7. Titer the helper phage produced. |
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| 使用说明 | |||||||||||||||||||
| 图谱 |
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| 产品名称 | VCSM13辅助噬菌体 | ||||||||||||||||||
| 商品货号 | TSPLA104469 | ||||||||||||||||||
| 使用说明 | |||||||||||||||||||
| 图谱 | |||||||||||||||||||
| 详情 |
产品名称 VCSM13辅助噬菌体 产品用途 噬菌体表面展示系统辅助噬菌体 产品特性 产生用于测序和突变的单链噬菌粒DNA 产品简介 VCSM13是M13KO7的衍生物，最初由Stratagene商业化，常用于噬菌体展示。VCSM13的表型尚未发表，确切序列尚不清楚。为了繁殖和扩增VCSM13辅助噬菌体，需要使用TG1、ER2738或菌株XL1 Blue MRF′大肠杆菌菌株，因为这些菌株含有supE44突变，该突变提供谷氨酰胺抑制剂tRNA。VCSM13辅助噬菌体严格用于从已切除的噬菌体中产生单链DNA（这也是M13KO7辅助噬菌体的用途）。 规格 1ml/管，1管 贮存 Recommends storing them at -80℃ with 50% Glycerol. As long as there is a -80℃ stock in the lab, the amplified lab prep can be stored at +4℃. 扩增 1. Streak out TG1 onto an 2YT plate. 2. Pick a single colony of TG1 and inoculate into 5ml 2×YT and grow until OD600 = 0.3 (~2.5×108 cells/ml). 3. Add helper phage at a multiplicity of infection (MOI) of 20:1 (phage-to-cells ratio). Note: If amplifying VCSM13 helper phage, add Kanamycin to a final concentration of 25μg/ml to the medium 30 minutes after the helper phage and cells have been allowed to grow together. 4. Grow the culture at 37℃ with vigorous aeration (~300rpm) for 8 hours. 5. Heat the culture to 65℃ for 15 minutes. 6. Spin down the cell debris and transfer the supernatant to a fresh tube. 7. Titer the helper phage produced. |
