| 产品名称： | Chlamydia trachomatis |
|---|---|
| 商品货号： | TS992291 |
| Classification： | Chlamydiaceae, Chlamydia |
| Agent： | |
| Strain： | Trachoma type J strain UW-36/Cx |
| Applications： | |
| Biosafety Level： | 2 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | J CommentsFBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. Antigenically related but distinct from type C. Disrupt cells of seed stock using glass beads and vortex. Infect shell vial monolayer with 0.1 mL of diluted seed stock. contrifuge at 2000X for 1 hr. Feed with medium containing cycloheximide. This preparation has tested negative for Mycoplasma by culture; the PCR test results are ambiguous. Antigenically related but distinct from type C. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC CRL-1696) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Name of Depositor： | ATCC |
| Special Collection： | ATCC |
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| 产品名称： | Chlamydia trachomatis |
|---|---|
| 商品货号： | TS992291 |
| Classification： | Chlamydiaceae, Chlamydia |
| Agent： | |
| Strain： | Trachoma type J strain UW-36/Cx |
| Applications： | |
| Biosafety Level： | 2 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | J CommentsFBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. Antigenically related but distinct from type C. Disrupt cells of seed stock using glass beads and vortex. Infect shell vial monolayer with 0.1 mL of diluted seed stock. contrifuge at 2000X for 1 hr. Feed with medium containing cycloheximide. This preparation has tested negative for Mycoplasma by culture; the PCR test results are ambiguous. Antigenically related but distinct from type C. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC CRL-1696) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Name of Depositor： | ATCC |
| Special Collection： | ATCC |
