pIB16.12-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pIB16.12
pIB16.12
规格:
货期:
编号:TS132000
品牌:Testobio
产品名称: pIB16.12
商品货号: TS132000
Designations: pIB16.12
GenBank Number:

M63838

Species: Homo sapiens, human
Depositors: JA Trapani
Vector:
Construct size (kb): 4.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IFI16
Other gene symbols: IFNFIP1
Genomic copy number: unique
Gene name: interferon, gamma-inducible protein 16
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 q22
Tissue: T lymphocyte, activated
Type of DNA: cDNA
Insert 5 end: Modification: linker
Insert 3 end:
Insert size (kb): 1.53
Cross references: DNA Seq. Acc.: M63838
Insert lengths(kb): 1.529999971389771
Tissue: T lymphocyte, activated
Gene product: interferon, gamma-inducible protein 16(interferon gamma-inducible protein 16) IFI16
Insert Size (kb): 1.530
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; HindIII--3.4, 1.1; PstI--3.0, 1.5; PvuII--2.5, 1.2, 0.7; SacI--4.5.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.32; HindIII--0.79; PvuII--1.29.
Corresponds to nt 403-1933 of the published sequence (encoding aa 135-644).
Detects a transcript of 2.7 kb in lymphoid cells.
References:

Trapani JA, et al. A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-gamma in myeloid cells. Immunogenetics 36: 369-376, 1992. PubMed: 1526658

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pIB16.12

  • 货号: TS132000
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pIB16.12
商品货号: TS132000
Designations: pIB16.12
GenBank Number:

M63838

Species: Homo sapiens, human
Depositors: JA Trapani
Vector:
Construct size (kb): 4.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IFI16
Other gene symbols: IFNFIP1
Genomic copy number: unique
Gene name: interferon, gamma-inducible protein 16
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 q22
Tissue: T lymphocyte, activated
Type of DNA: cDNA
Insert 5 end: Modification: linker
Insert 3 end:
Insert size (kb): 1.53
Cross references: DNA Seq. Acc.: M63838
Insert lengths(kb): 1.529999971389771
Tissue: T lymphocyte, activated
Gene product: interferon, gamma-inducible protein 16(interferon gamma-inducible protein 16) IFI16
Insert Size (kb): 1.530
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; HindIII--3.4, 1.1; PstI--3.0, 1.5; PvuII--2.5, 1.2, 0.7; SacI--4.5.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.32; HindIII--0.79; PvuII--1.29.
Corresponds to nt 403-1933 of the published sequence (encoding aa 135-644).
Detects a transcript of 2.7 kb in lymphoid cells.
References:

Trapani JA, et al. A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-gamma in myeloid cells. Immunogenetics 36: 369-376, 1992. PubMed: 1526658

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