Sappinia pedata Dangeard-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Sappinia pedata Dangeard
Sappinia pedata Dangeard
规格:
货期:
编号:TS132264
品牌:Testobio
产品名称： Sappinia pedata Dangeard
商品货号： TS132264
Strain Designations： AUK06-2-1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Isolated by MW Brown, Ground Litter Plant material, Auckland Island, New Zealand, 2006.
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Medium： ATCC® Medium 2432: wMY (weak Malt Yeast Extract)
Growth Conditions： Temperature: 15°C to 20°C
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Fresh growth medium w/o bacteria, 8.5 mL

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL ATCC medium 5080 (Dryls solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 104/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 2432.xa0 Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 15-20°C.
  10. Follow the protocol for maintenance of culture.
Name of Depositor： FW Spiegel
Year of Origin： 2006
References：

Brown MW, Spiegel FW, and Silberman JD. 2007. Amoeba at attention: phylogenetic affinity of Sappinia pedata. J. Eukaryot. Microbiol. 54:511-519. PubMed: 18070328

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Sappinia pedata Dangeard

  • 货号: TS132264
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Sappinia pedata Dangeard
商品货号： TS132264
Strain Designations： AUK06-2-1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Isolated by MW Brown, Ground Litter Plant material, Auckland Island, New Zealand, 2006.
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Medium： ATCC® Medium 2432: wMY (weak Malt Yeast Extract)
Growth Conditions： Temperature: 15°C to 20°C
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Fresh growth medium w/o bacteria, 8.5 mL

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL ATCC medium 5080 (Dryls solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 104/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 2432.xa0 Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 15-20°C.
  10. Follow the protocol for maintenance of culture.
Name of Depositor： FW Spiegel
Year of Origin： 2006
References：

Brown MW, Spiegel FW, and Silberman JD. 2007. Amoeba at attention: phylogenetic affinity of Sappinia pedata. J. Eukaryot. Microbiol. 54:511-519. PubMed: 18070328

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