pB8 [lambdaB1]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pB8 [lambdaB1]
pB8 [lambdaB1]
规格:
货期:
编号:TS132276
品牌:Testobio
产品名称: pB8 lambdaB1
商品货号: TS132276
Designations: pB8 lambdaB1
GenBank Number:

J02775

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.199999809265137
Insert:
DNA: cDNA
Insert lengths(kb): 1.600000023841858
Tissue: hepatocellular carcinoma Hep G2 cell line
Gene product: apolipoprotein B (including Ag(x) antigen) APOB
Alleles: B1, B3, B5, D2, B2, B4, D1
Insert Size (kb): 1.600
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): PstI--4.2, EcoRI/PstI--2.7, 1.6; EcoRI--4.2; HindIII--3.4, 0.7 doublet; PvuII--2.3, 1.3, 0.67.
Detects the hypervariable VNTR RFLP of the AT-rich minisatellite at the 3 end of APOB when genomic DNA is digested with HindIII and MspI but not with XbaI.
Insert ends are from linkers and are not present in the cDNA itself.
References:

Hegele RA, et al. Apolipoprotein B-gene DNA polymorphisms associated with myocardial infarction. N. Engl. J. Med. 315: 1509-1515, 1986. PubMed: 3024002

Huang LS, et al. Human apolipoprotein B cDNA clone isolation and demonstration that liver apolipoprotein B mRNA is 22 kilobases in length. Proc. Natl. Acad. Sci. USA 82: 6825-6829, 1985. PubMed: 2995989

Huang LS, Breslow JL. A unique AT-rich hypervariable minisatellite 3 to the ApoB gene defines a high information restriction fragment length polymorphism. J. Biol. Chem. 262: 8952-8955, 1987. PubMed: 2885324

Jan L Breslow, personal communication

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pB8 [lambdaB1]

  • 货号: TS132276
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pB8 lambdaB1
商品货号: TS132276
Designations: pB8 lambdaB1
GenBank Number:

J02775

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.199999809265137
Insert:
DNA: cDNA
Insert lengths(kb): 1.600000023841858
Tissue: hepatocellular carcinoma Hep G2 cell line
Gene product: apolipoprotein B (including Ag(x) antigen) APOB
Alleles: B1, B3, B5, D2, B2, B4, D1
Insert Size (kb): 1.600
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): PstI--4.2, EcoRI/PstI--2.7, 1.6; EcoRI--4.2; HindIII--3.4, 0.7 doublet; PvuII--2.3, 1.3, 0.67.
Detects the hypervariable VNTR RFLP of the AT-rich minisatellite at the 3 end of APOB when genomic DNA is digested with HindIII and MspI but not with XbaI.
Insert ends are from linkers and are not present in the cDNA itself.
References:

Hegele RA, et al. Apolipoprotein B-gene DNA polymorphisms associated with myocardial infarction. N. Engl. J. Med. 315: 1509-1515, 1986. PubMed: 3024002

Huang LS, et al. Human apolipoprotein B cDNA clone isolation and demonstration that liver apolipoprotein B mRNA is 22 kilobases in length. Proc. Natl. Acad. Sci. USA 82: 6825-6829, 1985. PubMed: 2995989

Huang LS, Breslow JL. A unique AT-rich hypervariable minisatellite 3 to the ApoB gene defines a high information restriction fragment length polymorphism. J. Biol. Chem. 262: 8952-8955, 1987. PubMed: 2885324

Jan L Breslow, personal communication

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