Crypthecodinium cohnii (Seligo) Javornicky-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Crypthecodinium cohnii (Seligo) Javornicky
Crypthecodinium cohnii (Seligo) Javornicky
规格:
货期:
编号:TS132365
品牌:Testobio
产品名称： Crypthecodinium cohnii (Seligo) Javornicky
商品货号： TS132365
Strain Designations： MB 3
Application：
Biofuel production
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Sargassum sp., Miami Beach, FL, 1977
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain： no
Comments：
Major Sibling Species Y
Medium： ATCC® Medium 460: A2E6 medium
Growth Conditions：
Temperature: 20°C to 25°C
Culture System:xa0Axenic
Cryopreservation：

Harvest and Preservation

  1. Harvest cells from cultures which are at or near peak density.xa0 Aseptically transfer cells to 15 mL plastic centrifuge tubes and centrifuge at ~150 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106/mL with fresh medium, then dilute to half this concentration by adding an equal amount of a 15% (v/v) sterile glycerol solution in freshxa0 medium.
  3. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).xa0 The time from mixing of the cell suspension and the glycerol solution, before the cooling cycle begins, should be no less than 15 min and no greater than 30 min.
  4. Place vials in a controlled-rate freezing unit. From room temperature, cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0xa0
  5. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.
  6. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
  7. Immediately after thawing, do not leave in the water bath; aseptically remove the contents of the ampule and transfer to a fresh test tube (or T-25 flask) containing 5 mL of ATCC Medium 460. Incubate culture at 20-25°C (test tubes are incubated upright with the cap loosened one-half turn).xa0 Subculture every 10-14d.
Name of Depositor： CA Beam, M Himes
Year of Origin： 1977
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Crypthecodinium cohnii (Seligo) Javornicky

  • 货号: TS132365
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Crypthecodinium cohnii (Seligo) Javornicky
商品货号： TS132365
Strain Designations： MB 3
Application：
Biofuel production
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Sargassum sp., Miami Beach, FL, 1977
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain： no
Comments：
Major Sibling Species Y
Medium： ATCC® Medium 460: A2E6 medium
Growth Conditions：
Temperature: 20°C to 25°C
Culture System:xa0Axenic
Cryopreservation：

Harvest and Preservation

  1. Harvest cells from cultures which are at or near peak density.xa0 Aseptically transfer cells to 15 mL plastic centrifuge tubes and centrifuge at ~150 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106/mL with fresh medium, then dilute to half this concentration by adding an equal amount of a 15% (v/v) sterile glycerol solution in freshxa0 medium.
  3. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).xa0 The time from mixing of the cell suspension and the glycerol solution, before the cooling cycle begins, should be no less than 15 min and no greater than 30 min.
  4. Place vials in a controlled-rate freezing unit. From room temperature, cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0xa0
  5. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.
  6. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
  7. Immediately after thawing, do not leave in the water bath; aseptically remove the contents of the ampule and transfer to a fresh test tube (or T-25 flask) containing 5 mL of ATCC Medium 460. Incubate culture at 20-25°C (test tubes are incubated upright with the cap loosened one-half turn).xa0 Subculture every 10-14d.
Name of Depositor： CA Beam, M Himes
Year of Origin： 1977
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