| 产品名称： | pDdeM1.6 |
|---|---|
| 商品货号： | TS132682 |
| Designations： | pDdeM1.6 |
| GenBank Number： | Y00449 |
| Species： | Desulfovibrio desulfuricans (Beijerinck) Kluyver and van Niel |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | produces protein modification methylase DdeI produces protein restriction endonuclease DdeI |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: modification methylase DdeI hsdM Target Gene: modification methylase DdeI, restriction endonuclease DdeI |
| Insert Size (kb)： | 2.300 |
| Media： | LB plus ampicillin (100 mcg/ml) plus chloramphenicol (30 mcg/ml)
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of pDdeM1.6 give the following sizes (kb): BamHI--5.2, 1.3; BglII--6.6; ClaI/HindIII--4.4, 1.9; EcoRI--6.6; HindIII--6.4. Restriction digests of pDdeR2.3 give the following sizes (kb): BamHI--4.4, 2.25; BglII--6.6; ClaI/HindIII--5.2, 1.4; EcoRI--6.6; HindIII--5.4, 1.4. The pDdeM1.6 insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.8; BglII--1.0. The 2.3 kb BamHI fragment contains all of the endonuclease and part of the methylase. Both are transcribed in the same direction. pDdeM1.6 encodes a truncated methylase that lacks 33 amino acids from the carboxy terminus and that are replaced by 6 amino acids from pBR322. This protein shows higher activity and stability than the wild type product. Avoid overgrowth and storage of cultures. |
| References： | Brooks JE, ODonnell KH. Method for producing the DDEI restriction endonuclease and methylase. US Patent 5,354,680 dated Oct 11 1994 Howard KA, et al. Cloning the DdeI restriction-modification system using a two-step method. Nucleic Acids Res. 14: 7939-7951, 1986. PubMed: 3022241 Sznyter LA, et al. Nucleotide sequence of the DdeI restriction-modification system and haracterization of the methylase protein. Nucleic Acids Res. 15: 8249-8266, 1987. PubMed: 2823226 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pDdeM1.6 |
|---|---|
| 商品货号： | TS132682 |
| Designations： | pDdeM1.6 |
| GenBank Number： | Y00449 |
| Species： | Desulfovibrio desulfuricans (Beijerinck) Kluyver and van Niel |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | produces protein modification methylase DdeI produces protein restriction endonuclease DdeI |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: modification methylase DdeI hsdM Target Gene: modification methylase DdeI, restriction endonuclease DdeI |
| Insert Size (kb)： | 2.300 |
| Media： | LB plus ampicillin (100 mcg/ml) plus chloramphenicol (30 mcg/ml)
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of pDdeM1.6 give the following sizes (kb): BamHI--5.2, 1.3; BglII--6.6; ClaI/HindIII--4.4, 1.9; EcoRI--6.6; HindIII--6.4. Restriction digests of pDdeR2.3 give the following sizes (kb): BamHI--4.4, 2.25; BglII--6.6; ClaI/HindIII--5.2, 1.4; EcoRI--6.6; HindIII--5.4, 1.4. The pDdeM1.6 insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.8; BglII--1.0. The 2.3 kb BamHI fragment contains all of the endonuclease and part of the methylase. Both are transcribed in the same direction. pDdeM1.6 encodes a truncated methylase that lacks 33 amino acids from the carboxy terminus and that are replaced by 6 amino acids from pBR322. This protein shows higher activity and stability than the wild type product. Avoid overgrowth and storage of cultures. |
| References： | Brooks JE, ODonnell KH. Method for producing the DDEI restriction endonuclease and methylase. US Patent 5,354,680 dated Oct 11 1994 Howard KA, et al. Cloning the DdeI restriction-modification system using a two-step method. Nucleic Acids Res. 14: 7939-7951, 1986. PubMed: 3022241 Sznyter LA, et al. Nucleotide sequence of the DdeI restriction-modification system and haracterization of the methylase protein. Nucleic Acids Res. 15: 8249-8266, 1987. PubMed: 2823226 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
