| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS132728 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | YPH98 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | Host strains for transformation, P. Hieter strains (BA1) |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Genotype： | MATa ura3-52 lys2-801(amber mutation) ade2-101(ochre mutation) leu2-delta1 trp1-delta1
Ref  Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436, (The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ .) |
| Preceptrol®： | no |
| Mating Type： | a |
| Ploidy： | haploid |
| Comments： | Host strains for transformation, P. Hieter strains (BA1) |
| Medium： | ATCC® Medium 1069: YPAD medium |
| Growth Conditions： | Temperature: 25.0°C Atmosphere: Typical aerobic |
| Name of Depositor： | YGSC |
| Special Collection： | Yeast Genetic Stock Center |
| Chain of Custody： | ATCC < |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ . Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ . |
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| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS132728 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | YPH98 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | Host strains for transformation, P. Hieter strains (BA1) |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Genotype： | MATa ura3-52 lys2-801(amber mutation) ade2-101(ochre mutation) leu2-delta1 trp1-delta1
RefSikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436, (The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ .) |
| Preceptrol®： | no |
| Mating Type： | a |
| Ploidy： | haploid |
| Comments： | Host strains for transformation, P. Hieter strains (BA1) |
| Medium： | ATCC® Medium 1069: YPAD medium |
| Growth Conditions： | Temperature: 25.0°C Atmosphere: Typical aerobic |
| Name of Depositor： | YGSC |
| Special Collection： | Yeast Genetic Stock Center |
| Chain of Custody： | ATCC < |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ . Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ . |
