| 产品名称： | pRK6 |
|---|---|
| 商品货号： | TS133450 |
| Designations： | pRK6 |
| GenBank Number： | J03745 |
| Species： | Homo sapiens, human |
| Depositors： | R Kaplan |
| Applications： | produces protein annexin V (endonexin II) |
| Vector： | Construct size (kb): 6.039999961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.445999979972839 Tissue: placenta Gene product: annexin V (endonexin II) ANX5 Alleles: A1, A1, A2, A2, B1, B1, B2, B2 Target Gene: annexin V (endonexin II) |
| Insert Size (kb)： | 1.446 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.3, 1.6; HindIII--5.6; PstI/HindIII--4.7, 1.2. The gene product expressed in bacteria demonstrates Ca++ dependent binding to phosphatidylserine-containing vesicles similar to the binding by placental endonexin II. Detects a 1.6 kb mRNA which is abundant in endothelial cells. The depositor indicates that pRK6 is identical to pRK1, except that the host strain for pRK6 is TGI and pRK1 is JM103. Sequence of the insert extends from the NcoI site including the ATG initiation signal through the HindIII site 323 bp downstream of the translational stop codon. An inducible trc promoter and a transcription terminator is provided by the vector. Enzyme(s) not detecting polymorphism: AccI, AvaI, BamHI, BanI, BanII, Eco109, EcoRV, HincII, HindIII, KpnI, MspI, RsaI, SspI, StyI, XbaI, XhoI. |
| References： | Kaplan R, et al. Cloning and expression of cDNA for human endonexin II, a Ca2+ and phospholipid binding protein. J. Biol. Chem. 263: 8037-8043, 1988. PubMed: 2967291 Ritty TM, et al. EcoRI and PvuII RFLPs in the endonexin II/annexin V (ANX5) gene on chromosome four. Nucleic Acids Res. 19: 1723, 1991. PubMed: 1674140 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pRK6 |
|---|---|
| 商品货号： | TS133450 |
| Designations： | pRK6 |
| GenBank Number： | J03745 |
| Species： | Homo sapiens, human |
| Depositors： | R Kaplan |
| Applications： | produces protein annexin V (endonexin II) |
| Vector： | Construct size (kb): 6.039999961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.445999979972839 Tissue: placenta Gene product: annexin V (endonexin II) ANX5 Alleles: A1, A1, A2, A2, B1, B1, B2, B2 Target Gene: annexin V (endonexin II) |
| Insert Size (kb)： | 1.446 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.3, 1.6; HindIII--5.6; PstI/HindIII--4.7, 1.2. The gene product expressed in bacteria demonstrates Ca++ dependent binding to phosphatidylserine-containing vesicles similar to the binding by placental endonexin II. Detects a 1.6 kb mRNA which is abundant in endothelial cells. The depositor indicates that pRK6 is identical to pRK1, except that the host strain for pRK6 is TGI and pRK1 is JM103. Sequence of the insert extends from the NcoI site including the ATG initiation signal through the HindIII site 323 bp downstream of the translational stop codon. An inducible trc promoter and a transcription terminator is provided by the vector. Enzyme(s) not detecting polymorphism: AccI, AvaI, BamHI, BanI, BanII, Eco109, EcoRV, HincII, HindIII, KpnI, MspI, RsaI, SspI, StyI, XbaI, XhoI. |
| References： | Kaplan R, et al. Cloning and expression of cDNA for human endonexin II, a Ca2+ and phospholipid binding protein. J. Biol. Chem. 263: 8037-8043, 1988. PubMed: 2967291 Ritty TM, et al. EcoRI and PvuII RFLPs in the endonexin II/annexin V (ANX5) gene on chromosome four. Nucleic Acids Res. 19: 1723, 1991. PubMed: 1674140 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
