Trypanosoma cruzi Chagas-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
Trypanosoma cruzi Chagas
Trypanosoma cruzi Chagas
规格:
货期:
编号:TS133485
品牌:Testobio
产品名称： Trypanosoma cruzi Chagas
商品货号： TS133485
Strain Designations： CA-I CL72 Lampit Resistant
Application：
Vector borne research
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
Lampit (=Nifurtimox) resistant strain derived from CA-I CL72 (=ATCC 50791)
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Medium： ATCC® Medium 1029: LIT medium
Growth Conditions：
Temperature: 20-25°C
Cryopreservation：
  1. Harvest cells from several cultures in very late logarithmic to early stationary phase of growth.xa0 Vigorously agitate to suspend the cells.
  2. Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.
  3. Centrifuge at ~800 x g for 5 min.
  4. While cells are centrifuging, prepare a 10% solution of DMSO in complete ATCC Medium 1029.xa0 Cool on ice.
  5. Remove the supernatant and pool the cell pellets to the final volume desired with fresh growth medium.
  6. Combine the cell suspension with an equal volume of 10% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 5% DMSO.
  7. Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).
  8. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 At -40°C, plunge ampules into liquid nitrogen.xa0 Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.). xa0
  9. Store ampules in a liquid nitrogen refrigerator until needed.
  10. To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.xa0 Allow the ampule to thaw completely (2-3 min).
  11. Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh complete ATCC medium 1029.
  12. Screw the cap on tightly and incubate at 20-25°C.xa0xa0xa0 Observe the culture daily and transfer when numerous trophozoites are observed.xa0xa0xa0xa0xa0xa0xa0xa0xa0
Name of Depositor： JA Dvorak
Special Collection： NCRR Contract
References：

Nozaki T, et al. Cellular and molecular biological analyses of nifurtimox resistance in Trypanosoma cruzi. Am. J. Trop. Med. Hyg. 55: 111-117, 1996. PubMed: 8702014

首页 > 产品中心 > 微生物培养 > 菌株 > null > Trypanosoma cruzi Chagas

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

Trypanosoma cruzi Chagas

  • 货号: TS133485
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Trypanosoma cruzi Chagas
商品货号： TS133485
Strain Designations： CA-I CL72 Lampit Resistant
Application：
Vector borne research
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
Lampit (=Nifurtimox) resistant strain derived from CA-I CL72 (=ATCC 50791)
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Medium： ATCC® Medium 1029: LIT medium
Growth Conditions：
Temperature: 20-25°C
Cryopreservation：
  1. Harvest cells from several cultures in very late logarithmic to early stationary phase of growth.xa0 Vigorously agitate to suspend the cells.
  2. Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.
  3. Centrifuge at ~800 x g for 5 min.
  4. While cells are centrifuging, prepare a 10% solution of DMSO in complete ATCC Medium 1029.xa0 Cool on ice.
  5. Remove the supernatant and pool the cell pellets to the final volume desired with fresh growth medium.
  6. Combine the cell suspension with an equal volume of 10% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 5% DMSO.
  7. Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).
  8. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 At -40°C, plunge ampules into liquid nitrogen.xa0 Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.). xa0
  9. Store ampules in a liquid nitrogen refrigerator until needed.
  10. To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.xa0 Allow the ampule to thaw completely (2-3 min).
  11. Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh complete ATCC medium 1029.
  12. Screw the cap on tightly and incubate at 20-25°C.xa0xa0xa0 Observe the culture daily and transfer when numerous trophozoites are observed.xa0xa0xa0xa0xa0xa0xa0xa0xa0
Name of Depositor： JA Dvorak
Special Collection： NCRR Contract
References：

Nozaki T, et al. Cellular and molecular biological analyses of nifurtimox resistance in Trypanosoma cruzi. Am. J. Trop. Med. Hyg. 55: 111-117, 1996. PubMed: 8702014

扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: