| 产品名称： | p11-2-2 |
|---|---|
| 商品货号： | TS133596 |
| Designations： | p11-2-2 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: genomic Insert lengths(kb): 0.6000000238418579 Gene product: DNA Segment, single copy D12S6 Alleles: A1, A1, A2, A2, C1, C2, D1, D2 |
| Insert Size (kb)： | 0.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul, |
| Comments： | Constructed by inserting Sau3A fragment of cosmid 1-11 into BamHI site. Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 0.6; PstI--5.0, 1.0; BamHI--uncut; SalI--6.0; HindIII--6.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The probe hybridizes to the inserted sequence of the p11-1-111 RFLP and thus fails to hybridize to chromosomes lacking this sequence. |
| References： | Buroker NE, et al. Four restriction length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. Hum. Genet. 72: 86-94, 1986. PubMed: 3002956 OConnell P, et al. A primary genetic linkage map for human chromosome 12. Genomics 1: 93-102, 1987. PubMed: 3478299 Michael Litt, personal communication |
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| 产品名称： | p11-2-2 |
|---|---|
| 商品货号： | TS133596 |
| Designations： | p11-2-2 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: genomic Insert lengths(kb): 0.6000000238418579 Gene product: DNA Segment, single copy D12S6 Alleles: A1, A1, A2, A2, C1, C2, D1, D2 |
| Insert Size (kb)： | 0.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul, |
| Comments： | Constructed by inserting Sau3A fragment of cosmid 1-11 into BamHI site. Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 0.6; PstI--5.0, 1.0; BamHI--uncut; SalI--6.0; HindIII--6.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The probe hybridizes to the inserted sequence of the p11-1-111 RFLP and thus fails to hybridize to chromosomes lacking this sequence. |
| References： | Buroker NE, et al. Four restriction length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. Hum. Genet. 72: 86-94, 1986. PubMed: 3002956 OConnell P, et al. A primary genetic linkage map for human chromosome 12. Genomics 1: 93-102, 1987. PubMed: 3478299 Michael Litt, personal communication |
