pHTalpha2 [UTR-01255, WI-7091]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pHTalpha2 [UTR-01255, WI-7091]
pHTalpha2 [UTR-01255, WI-7091]
规格:
货期:
编号:TS133614
品牌:Testobio
产品名称: pHTalpha2 UTR-01255, WI-7091
商品货号: TS133614
Designations: pHTalpha2 UTR-01255, WI-7091
GenBank Number:

X51420

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.800000190734863
Insert:
DNA: cDNA
Insert lengths(kb): 2.799999952316284
Tissue: melanoma MeWo cell line
Gene product: tyrosinase-related protein 1(homolog of b-locus protein, melanoma antigen gp 75) CAS2
Alleles: A2, A2, A1, A1, A2, A1
Insert Size (kb): 2.800
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; BglII--5.8; AvaI--5.8; HincII--3.4, 2.4; PstI/PvuII--1.6, 1.5, 1.0, 0.8, 0.4.
This clone, which was not derived from the same cell line as the published sequence (S7), does not have the HindIII site at nucleotide 748. This is probably due to polymorphism. It does have a BamHI site at 929.
Either an AvaI/NdeI or an SmaI/NdeI digest gives a 1.7 kb fragment containing the protein-coding region except for a putative signal peptide.
The vector lacks the SV40 promoter.
This is a full length cDNA clone; coding sequences extend from nucleotide 130 through 1710.
References:

Cohen T, et al. Nucleotide sequence of the cDNA encoding human tyrosinase-related protein. Nucleic Acids Res. 18: 2807-2808, 1990. PubMed: 2111010

Shigeki Shibahara, personal communication

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pHTalpha2 [UTR-01255, WI-7091]

  • 货号: TS133614
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pHTalpha2 UTR-01255, WI-7091
商品货号: TS133614
Designations: pHTalpha2 UTR-01255, WI-7091
GenBank Number:

X51420

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.800000190734863
Insert:
DNA: cDNA
Insert lengths(kb): 2.799999952316284
Tissue: melanoma MeWo cell line
Gene product: tyrosinase-related protein 1(homolog of b-locus protein, melanoma antigen gp 75) CAS2
Alleles: A2, A2, A1, A1, A2, A1
Insert Size (kb): 2.800
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; BglII--5.8; AvaI--5.8; HincII--3.4, 2.4; PstI/PvuII--1.6, 1.5, 1.0, 0.8, 0.4.
This clone, which was not derived from the same cell line as the published sequence (S7), does not have the HindIII site at nucleotide 748. This is probably due to polymorphism. It does have a BamHI site at 929.
Either an AvaI/NdeI or an SmaI/NdeI digest gives a 1.7 kb fragment containing the protein-coding region except for a putative signal peptide.
The vector lacks the SV40 promoter.
This is a full length cDNA clone; coding sequences extend from nucleotide 130 through 1710.
References:

Cohen T, et al. Nucleotide sequence of the cDNA encoding human tyrosinase-related protein. Nucleic Acids Res. 18: 2807-2808, 1990. PubMed: 2111010

Shigeki Shibahara, personal communication

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