| 产品名称： | cDNA 210 |
|---|---|
| 商品货号： | TS133803 |
| Designations： | cDNA 210 |
| GenBank Number： | X63546 |
| Species： | Homo sapiens, human |
| Depositors： | M Hill |
| Applications： | The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe. |
| Vector： | Construct size (kb): 9.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 6.300000190734863 Tissue: DNA from ED1 cell line (tre-transfected NIH 3T3) Gene product: tre-2 oncogene(tre-2 oncogene) TRE2* |
| Insert Size (kb)： | 6.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.1, 2.4, 0.46; PstI--7.6, 1.45, 0.72; HindIII--5.2, 4.5; PvuII--2.6, 2.5, 1.10, 0.82, 0.74 (doublet), 0.49 (doublet); SalI--9.6. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.41, 3.96; PstI--0.67, 2.10; HindIII--1.92. Contains two complete open reading frames, from nt 112-2472 and from nt 2460-4220 of the insert. The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe. It detects mRNA of 8.2 kb in Ewings sarcoma cells (the source of the transfected DNA) and other neoplasms. Corresponds to nt 1586 - 7878 of the sequence record. |
| References： | Nakamura T, et al. A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. Oncogene 7: 733-741, 1992. PubMed: 1565468 Nakamura T, et al. Nucleotide sequence of mouse L19 ribosomal protein cDNA isolated in screening with tre oncogene probes. DNA Cell Biol. 9: 697-703, 1990. PubMed: 1702292 |
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| 产品名称： | cDNA 210 |
|---|---|
| 商品货号： | TS133803 |
| Designations： | cDNA 210 |
| GenBank Number： | X63546 |
| Species： | Homo sapiens, human |
| Depositors： | M Hill |
| Applications： | The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe. |
| Vector： | Construct size (kb): 9.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 6.300000190734863 Tissue: DNA from ED1 cell line (tre-transfected NIH 3T3) Gene product: tre-2 oncogene(tre-2 oncogene) TRE2* |
| Insert Size (kb)： | 6.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.1, 2.4, 0.46; PstI--7.6, 1.45, 0.72; HindIII--5.2, 4.5; PvuII--2.6, 2.5, 1.10, 0.82, 0.74 (doublet), 0.49 (doublet); SalI--9.6. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.41, 3.96; PstI--0.67, 2.10; HindIII--1.92. Contains two complete open reading frames, from nt 112-2472 and from nt 2460-4220 of the insert. The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe. It detects mRNA of 8.2 kb in Ewings sarcoma cells (the source of the transfected DNA) and other neoplasms. Corresponds to nt 1586 - 7878 of the sequence record. |
| References： | Nakamura T, et al. A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. Oncogene 7: 733-741, 1992. PubMed: 1565468 Nakamura T, et al. Nucleotide sequence of mouse L19 ribosomal protein cDNA isolated in screening with tre oncogene probes. DNA Cell Biol. 9: 697-703, 1990. PubMed: 1702292 |
