| 产品名称： | Reovirus 3 |
|---|---|
| 商品货号： | TS133869 |
| Classification： | Reoviridae, Orthoreovirus, Mammalian orthoreovirus |
| Deposited As： | Reovirus type 3 |
| Agent： | Reovirus 3 |
| Strain： | Dearing |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Hemagglutinates with RBC (Gomatos PJ, Tamm, I. Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17:455-461, 1962. PubMed: 13900038). |
| Effect on Host： | CPE, granulation and sloughing |
| Recommended Host： |
LLC-MK2 Derivative (ATCC® CCL-7.1™)
|
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-5 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | WA Chappell |
| Source： | Child with diarrhea |
| References： | Ramos-Alvarez M, Sabin AB. Enteropathogenic viruses and bacteria; role in summer diarrheal diseases of infancy and early childhood. J. Am. Med. Assoc. 167: 147-156, 1958. PubMed: 13538681 Gomatos PJ, Tamm, I. Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17:455-461, 1962. PubMed: 13900038 |
| Cross References： | Nucleotide (GenBank) : AJ133091 Reovirus type 3 S2 gene, strain Dearing. |
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| 产品名称： | Reovirus 3 |
|---|---|
| 商品货号： | TS133869 |
| Classification： | Reoviridae, Orthoreovirus, Mammalian orthoreovirus |
| Deposited As： | Reovirus type 3 |
| Agent： | Reovirus 3 |
| Strain： | Dearing |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | Hemagglutinates with RBC (Gomatos PJ, Tamm, I. Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17:455-461, 1962. PubMed: 13900038). |
| Effect on Host： | CPE, granulation and sloughing |
| Recommended Host： |
LLC-MK2 Derivative (ATCC® CCL-7.1™)
|
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-5 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | WA Chappell |
| Source： | Child with diarrhea |
| References： | Ramos-Alvarez M, Sabin AB. Enteropathogenic viruses and bacteria; role in summer diarrheal diseases of infancy and early childhood. J. Am. Med. Assoc. 167: 147-156, 1958. PubMed: 13538681 Gomatos PJ, Tamm, I. Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17:455-461, 1962. PubMed: 13900038 |
| Cross References： | Nucleotide (GenBank) : AJ133091 Reovirus type 3 S2 gene, strain Dearing. |
