pHP11 [UTR-9853]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pHP11 [UTR-9853]
pHP11 [UTR-9853]
规格:
货期:
编号:TS134194
品牌:Testobio
产品名称: pHP11 UTR-9853
商品货号: TS134194
Designations: pHP11 UTR-9853
GenBank Number:

M81457

Species: Homo sapiens, human
Vector:
Construct size (kb): 3.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAL1L
Gene name: calpactin I, light polypeptide (p11)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1, cR
Tissue: foreskin keratinocyte
Type of DNA: cDNA
Insert 5 end: EcoRI
Insert 3 end: HindIII
Insert size (kb): 0.55
Cross references: DNA Seq. Acc.: M81457
Nucleotides 1-545 of the insert correspond to
nucleotides 1-545 of M81457.
Insert lengths(kb): 0.550000011920929
Tissue: foreskin keratinocyte
Gene product: calpactin I, light polypeptide (p11) CAL1L
Insert Size (kb): 0.550
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.6; ScaI--2.2, 1.5; NcoI--3.6; XmnI--1.9, 1.5; BamHI--3.5.
The insert contains the following restriction sites (approximate kb from the 5 end): NcoI--0.1; ScaI--0.17; SphI--0.32; XmnI--0.46.
Corresponds to nt 1-545 of the sequence record, including the complete coding region (nt 72-365). The 5 end is linker derived; the 3 end is a naturally occurring site.
References:

Dooley TP, et al. cDNA sequence of human p11 calpactin I light chain. Genomics 13: 866-868, 1992. PubMed: 1386341

Thomas P Dooley, personal communication

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pHP11 [UTR-9853]

  • 货号: TS134194
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pHP11 UTR-9853
商品货号: TS134194
Designations: pHP11 UTR-9853
GenBank Number:

M81457

Species: Homo sapiens, human
Vector:
Construct size (kb): 3.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAL1L
Gene name: calpactin I, light polypeptide (p11)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1, cR
Tissue: foreskin keratinocyte
Type of DNA: cDNA
Insert 5 end: EcoRI
Insert 3 end: HindIII
Insert size (kb): 0.55
Cross references: DNA Seq. Acc.: M81457
Nucleotides 1-545 of the insert correspond to
nucleotides 1-545 of M81457.
Insert lengths(kb): 0.550000011920929
Tissue: foreskin keratinocyte
Gene product: calpactin I, light polypeptide (p11) CAL1L
Insert Size (kb): 0.550
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.6; ScaI--2.2, 1.5; NcoI--3.6; XmnI--1.9, 1.5; BamHI--3.5.
The insert contains the following restriction sites (approximate kb from the 5 end): NcoI--0.1; ScaI--0.17; SphI--0.32; XmnI--0.46.
Corresponds to nt 1-545 of the sequence record, including the complete coding region (nt 72-365). The 5 end is linker derived; the 3 end is a naturally occurring site.
References:

Dooley TP, et al. cDNA sequence of human p11 calpactin I light chain. Genomics 13: 866-868, 1992. PubMed: 1386341

Thomas P Dooley, personal communication

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