pPD39
pPD39
规格:
货期:
编号:TS134311
品牌:Testobio
| 产品名称： | pPD39 |
|---|---|
| 商品货号： | TS134311 |
| Designations： | pPD39 |
| GenBank Number： | U02043 |
| Species： | Homo sapiens, human |
| Depositors： | PL Deininger |
| Vector： | Construct size (kb): 3.299999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS+ Intact vector size: 2.964 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Genomic copy number: repetitive Gene name: Alu consensus sequence, repetitive Contains complete coding sequence?: U Type of DNA: genomic Insert end: Modification: BamHI linkers Insert end: Modification: BamHI linkers Insert size (kb): 0.33 Cross references: DNA Seq. Acc.: U02043 Insert lengths(kb): 0.3300000131130219 Gene product: Alu consensus sequence, repetitive |
| Insert Size (kb)： | 0.330 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 0.4; PvuI--2.3, 1.1; SacI--3.4; PvuII--2.6, 0.8; KpnI--3.4. A perfect HS consensus sequence (HSC3N1) modified at the ends (4 bp and 24 bp) for cloning. Hybridizes efficiently to a few Alu sequences in a mouse cell background at 52C in 50% formamide, with a wash at 61C. Because the sequence is closer to consensus than BLUR8, it matches rodent B1 family better with an increase in rodent background. |
| References： | Batzer MA, et al. A consensus Alu repeat probe for physical mapping. GATA 11: 34-38, 1994. PubMed: 7917498 Prescott L Deininger, personal communication |
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| 产品名称： | pPD39 |
|---|---|
| 商品货号： | TS134311 |
| Designations： | pPD39 |
| GenBank Number： | U02043 |
| Species： | Homo sapiens, human |
| Depositors： | PL Deininger |
| Vector： | Construct size (kb): 3.299999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS+ Intact vector size: 2.964 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Genomic copy number: repetitive Gene name: Alu consensus sequence, repetitive Contains complete coding sequence?: U Type of DNA: genomic Insert end: Modification: BamHI linkers Insert end: Modification: BamHI linkers Insert size (kb): 0.33 Cross references: DNA Seq. Acc.: U02043 Insert lengths(kb): 0.3300000131130219 Gene product: Alu consensus sequence, repetitive |
| Insert Size (kb)： | 0.330 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 0.4; PvuI--2.3, 1.1; SacI--3.4; PvuII--2.6, 0.8; KpnI--3.4. A perfect HS consensus sequence (HSC3N1) modified at the ends (4 bp and 24 bp) for cloning. Hybridizes efficiently to a few Alu sequences in a mouse cell background at 52C in 50% formamide, with a wash at 61C. Because the sequence is closer to consensus than BLUR8, it matches rodent B1 family better with an increase in rodent background. |
| References： | Batzer MA, et al. A consensus Alu repeat probe for physical mapping. GATA 11: 34-38, 1994. PubMed: 7917498 Prescott L Deininger, personal communication |
