pGEV-HIS3 plasmid in E. coli
pGEV-HIS3 plasmid in E. coli
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货期:
编号:TS134315
品牌:Testobio
| 产品名称： | pGEV-HIS3 plasmid in E. coli |
|---|---|
| 商品货号： | TS134315 |
| Designations： | pGEV-HIS3 plasmid in E. coli |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.4450000000000000 Vector: pGEV-HIS3 plasmid in E. coli (plasmid) Type: expression Promoters: Eukaryotic Promoter for expression MRP7 Construction: pRS313, MRP7 promoter, GEV (Gal4, B-estradiol activator, VP16) Marker(s):AmpR |
| Comments： | A 2.65 kb NotI - BglII fragment containing the MRP7 promoter and the GAL4-hER-VP16 construct was inserted into the NotI and BamHI sites of pRS313. The order of features in the insert are - MRP7 promoter (nt 1-1092) - GAL4 (a.a. 1-93) - Estradiol receptor-alpha (a.a. 282-576) - Herpes Simplex virus protein 16 (a.a. 424-490). The start codon begins at nt 1093. The stop codon begins at nt 2497. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of Escherichia coli GC10 containing the plasmid. |
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pGEV-HIS3 plasmid in E. coli
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| 产品名称： | pGEV-HIS3 plasmid in E. coli |
|---|---|
| 商品货号： | TS134315 |
| Designations： | pGEV-HIS3 plasmid in E. coli |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.4450000000000000 Vector: pGEV-HIS3 plasmid in E. coli (plasmid) Type: expression Promoters: Eukaryotic Promoter for expression MRP7 Construction: pRS313, MRP7 promoter, GEV (Gal4, B-estradiol activator, VP16) Marker(s):AmpR |
| Comments： | A 2.65 kb NotI - BglII fragment containing the MRP7 promoter and the GAL4-hER-VP16 construct was inserted into the NotI and BamHI sites of pRS313. The order of features in the insert are - MRP7 promoter (nt 1-1092) - GAL4 (a.a. 1-93) - Estradiol receptor-alpha (a.a. 282-576) - Herpes Simplex virus protein 16 (a.a. 424-490). The start codon begins at nt 1093. The stop codon begins at nt 2497. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of Escherichia coli GC10 containing the plasmid. |
