| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS134353 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | BY4718 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | transformation host with non-revertable mutations for auxotrophic markers transformation host with non-revertable designer deletions |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Genotype： | MATa met15delta0 ura3delta0
Ref  Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Preceptrol®： | no |
| Mating Type： | a |
| Ploidy： | haploid |
| Comments： | One of a series of strains (ATCC 200866-200902) useful for gene disruption experiments. The strains differ in mating type and in non-revertable mutations for one or more of these auxotrophic markers: ade2delta::hisG, his3delta200, leu2delta0, lys2delta0, met15delta0, trp1delta63, ura3delta0. |
| Morphology： | After 3 days on YEPD medium at 25°C, colonies are off-white, smooth, butyrous. Cells are ovoid or irregular; single, in pairs or small clusters. |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
No DNA sequencing was performed in house on this product.
|
| Name of Depositor： | JD Boeke |
| Special Collection： | NCRR Contract |
| References： | Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
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| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS134353 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | BY4718 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | transformation host with non-revertable mutations for auxotrophic markers transformation host with non-revertable designer deletions |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Genotype： | MATa met15delta0 ura3delta0
RefBrachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Preceptrol®： | no |
| Mating Type： | a |
| Ploidy： | haploid |
| Comments： | One of a series of strains (ATCC 200866-200902) useful for gene disruption experiments. The strains differ in mating type and in non-revertable mutations for one or more of these auxotrophic markers: ade2delta::hisG, his3delta200, leu2delta0, lys2delta0, met15delta0, trp1delta63, ura3delta0. |
| Morphology： | After 3 days on YEPD medium at 25°C, colonies are off-white, smooth, butyrous. Cells are ovoid or irregular; single, in pairs or small clusters. |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
No DNA sequencing was performed in house on this product.
|
| Name of Depositor： | JD Boeke |
| Special Collection： | NCRR Contract |
| References： | Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
