Leishmania major (Yakimoff and Schokhor) Bray et al.-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Leishmania major (Yakimoff and Schokhor) Bray et al.
Leishmania major (Yakimoff and Schokhor) Bray et al.
规格:
货期:
编号:TS134360
品牌:Testobio
产品名称： Leishmania major (Yakimoff and Schokhor) Bray et al.
商品货号： TS134360
Strain Designations： Seidman
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Medium： ATCC® Medium 2736: M199, Modified Medium
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 1012: Diphasic blood agar medium
ATCC® Medium 807: Brain heart infusion blood agar
Growth Conditions：
Temperature: 20-25°C
Cryopreservation：
  1. Harvest cells from a culture that is at or near peak density by centrifugation at ~800 x g for 5 min. Pool the cell pellets into a single tube.
  2. Adjust the concentration of cells to 2.0 x 107/ml.xa0 If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. Prepare a 10% (v/v) sterile DMSO solution in fresh medium as follows:xa0 Add the required volume of DMSO to a glass screw-capped test tube and place on ice.xa0 Allow the DMSO to solidify.xa0 Add the required volume of refrigerated medium.xa0 Dissolve the DMSO by inverting several times.xa0 If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 107 and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 60 min.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
  7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 10.0 ml of fresh ATCC medium 2736.
  10. Incubate the tube at 20-25°C with the cap screwed on tightly.
Name of Depositor： D Sacks
Chain of Custody：
D Sacks <-- F Neva
References：

Neva FA, et al. Cutaneous leishmaniasis--a case with persistent organisms after treatment in presence of normal immune response. Am. J. Trop. Med. Hyg. 28: 467-71, 1979. PubMed: 222157

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Leishmania major (Yakimoff and Schokhor) Bray et al.

  • 货号: TS134360
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Leishmania major (Yakimoff and Schokhor) Bray et al.
商品货号： TS134360
Strain Designations： Seidman
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Medium： ATCC® Medium 2736: M199, Modified Medium
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 1012: Diphasic blood agar medium
ATCC® Medium 807: Brain heart infusion blood agar
Growth Conditions：
Temperature: 20-25°C
Cryopreservation：
  1. Harvest cells from a culture that is at or near peak density by centrifugation at ~800 x g for 5 min. Pool the cell pellets into a single tube.
  2. Adjust the concentration of cells to 2.0 x 107/ml.xa0 If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. Prepare a 10% (v/v) sterile DMSO solution in fresh medium as follows:xa0 Add the required volume of DMSO to a glass screw-capped test tube and place on ice.xa0 Allow the DMSO to solidify.xa0 Add the required volume of refrigerated medium.xa0 Dissolve the DMSO by inverting several times.xa0 If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 107 and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 60 min.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
  7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 10.0 ml of fresh ATCC medium 2736.
  10. Incubate the tube at 20-25°C with the cap screwed on tightly.
Name of Depositor： D Sacks
Chain of Custody：
D Sacks <-- F Neva
References：

Neva FA, et al. Cutaneous leishmaniasis--a case with persistent organisms after treatment in presence of normal immune response. Am. J. Trop. Med. Hyg. 28: 467-71, 1979. PubMed: 222157

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