cPFKL3.0-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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cPFKL3.0
cPFKL3.0
规格:
货期:
编号:TS134489
品牌:Testobio
产品名称: cPFKL3.0
商品货号: TS134489
Designations: cPFKL3.0
GenBank Number:

X15573

Species: Homo sapiens, human
Vector:
Construct size (kb): 6.0
Insert:
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: liver
Gene product: phosphofructokinase, liver type( human liver-type 1-phosphofructokinase, Phosphofructokinase, liver type) PFKL
Alleles: A1, A2
Insert Size (kb): 3.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0 (doublet); BamHI--3.0, 2.0, 1.0; SalI--6.0; XhoI/PstI--3.0, 1.8, 0.55 (doublet); XbaI--3.0 (doublet).
This was constructed from 3 overlapping cDNAs. When transcribed and translated in vitro, a polypeptide of 80 kDa recognized by anti-PFKL antibodies is generated.
The clone detects the following genomic restriction fragments (kb), with those not from PFKL indicated by a *: BamHI--8.6, 7.5, 5.0, 4.2*, 3.9*, 3.3, 2.9, 2.2*; KpnI--14, 11, 4.2, 3.3*, 2.6; HindIII-- >20, 12.3, 5.2.
A fragment specific to PFKL can be generated from 3 sequences.
The insert contains the complete coding sequences, with 5 and 3 untranslated sequences.
The KpnI polymorphic site is located in the 3.3 kb BamHI genomic fragment .
References:

Elson A, et al. The structure of the human liver-type phosphofructokinase gene. Genomics 7: 47-56, 1990. PubMed: 2139864

Levanon D, et al. The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK. DNA 8: 733-743, 1989. PubMed: 2533063

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cPFKL3.0

  • 货号: TS134489
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: cPFKL3.0
商品货号: TS134489
Designations: cPFKL3.0
GenBank Number:

X15573

Species: Homo sapiens, human
Vector:
Construct size (kb): 6.0
Insert:
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: liver
Gene product: phosphofructokinase, liver type( human liver-type 1-phosphofructokinase, Phosphofructokinase, liver type) PFKL
Alleles: A1, A2
Insert Size (kb): 3.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0 (doublet); BamHI--3.0, 2.0, 1.0; SalI--6.0; XhoI/PstI--3.0, 1.8, 0.55 (doublet); XbaI--3.0 (doublet).
This was constructed from 3 overlapping cDNAs. When transcribed and translated in vitro, a polypeptide of 80 kDa recognized by anti-PFKL antibodies is generated.
The clone detects the following genomic restriction fragments (kb), with those not from PFKL indicated by a *: BamHI--8.6, 7.5, 5.0, 4.2*, 3.9*, 3.3, 2.9, 2.2*; KpnI--14, 11, 4.2, 3.3*, 2.6; HindIII-- >20, 12.3, 5.2.
A fragment specific to PFKL can be generated from 3 sequences.
The insert contains the complete coding sequences, with 5 and 3 untranslated sequences.
The KpnI polymorphic site is located in the 3.3 kb BamHI genomic fragment .
References:

Elson A, et al. The structure of the human liver-type phosphofructokinase gene. Genomics 7: 47-56, 1990. PubMed: 2139864

Levanon D, et al. The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK. DNA 8: 733-743, 1989. PubMed: 2533063

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