BL29-113-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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BL29-113
BL29-113
规格:
货期:
编号:TS134503
品牌:Testobio
产品名称: BL29-113
商品货号: TS134503
Designations: BL29-113
GenBank Number:

T25885

Species: Homo sapiens, human
Depositors: R Sanders
Vector:
Construct size (kb): 3.72000002861023
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, numerous copies, expressed
Contains complete coding sequence?: U
Tissue: BL29 cell line
Disease: Burkitts lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.82
Cross references: DNA Seq. Acc.: T25885; EST: EST113R; Primer: T3
Insert lengths(kb): 0.8199999928474426
Tissue: BL29 cell line Burkitts lymphoma
Disease: Burkitts lymphoma
Gene product: DNA Segment, numerous copies, expressed
Insert Size (kb): 0.820
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): PvuII--2.5, 0.86, 0.34; PstI--3.3, 0.45; HindIII--3.7; EcoRI--2.9, 0.6, 0.24; BamHI--3.7.
Maps to 8 different chromosomes.
References:

Rhiannon Sanders, personal communication

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BL29-113

  • 货号: TS134503
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: BL29-113
商品货号: TS134503
Designations: BL29-113
GenBank Number:

T25885

Species: Homo sapiens, human
Depositors: R Sanders
Vector:
Construct size (kb): 3.72000002861023
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, numerous copies, expressed
Contains complete coding sequence?: U
Tissue: BL29 cell line
Disease: Burkitts lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.82
Cross references: DNA Seq. Acc.: T25885; EST: EST113R; Primer: T3
Insert lengths(kb): 0.8199999928474426
Tissue: BL29 cell line Burkitts lymphoma
Disease: Burkitts lymphoma
Gene product: DNA Segment, numerous copies, expressed
Insert Size (kb): 0.820
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): PvuII--2.5, 0.86, 0.34; PstI--3.3, 0.45; HindIII--3.7; EcoRI--2.9, 0.6, 0.24; BamHI--3.7.
Maps to 8 different chromosomes.
References:

Rhiannon Sanders, personal communication

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