HFBCA08-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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HFBCA08
HFBCA08
规格:
货期:
编号:TS134721
品牌:Testobio
产品名称: HFBCA08
商品货号: TS134721
Designations: HFBCA08
GenBank Number:

M78324

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: D0S1312E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens synaptotagmin 1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: M78324; EST: EST00472; Primer: M13 Forward
DNA Seq. Acc.: T04843; EST: EST02730; Primer: M13 Reverse;
THC id: THC91295
DNA Seq. Acc.: unsubmitted; EST: EST07497; THC id: THC91295
Insert lengths(kb): 2.5
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: synaptotagmin 1(synaptotagmin (p65)) SYT
Insert Size (kb): 2.5
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4, 1.1; PstI--3.2, 1.5, 0.35 (doublet), 0.1; HindIII--4.55, 0.95.
The insert contains multiple EcoRI fragments (kb): 1.5, 1.1. It is important to note these may have been derived from different cDNAs.
EST00472 matches Swiss-Prot accession SY65$HUMAN with 53.57% identity and 82.14% similarity over the sequence range: 229-312.
This sequence record is for human synaptotagmin (p65) (SYT).
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

J C Venter, personal communication

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HFBCA08

  • 货号: TS134721
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: HFBCA08
商品货号: TS134721
Designations: HFBCA08
GenBank Number:

M78324

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: D0S1312E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens synaptotagmin 1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: M78324; EST: EST00472; Primer: M13 Forward
DNA Seq. Acc.: T04843; EST: EST02730; Primer: M13 Reverse;
THC id: THC91295
DNA Seq. Acc.: unsubmitted; EST: EST07497; THC id: THC91295
Insert lengths(kb): 2.5
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: synaptotagmin 1(synaptotagmin (p65)) SYT
Insert Size (kb): 2.5
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4, 1.1; PstI--3.2, 1.5, 0.35 (doublet), 0.1; HindIII--4.55, 0.95.
The insert contains multiple EcoRI fragments (kb): 1.5, 1.1. It is important to note these may have been derived from different cDNAs.
EST00472 matches Swiss-Prot accession SY65$HUMAN with 53.57% identity and 82.14% similarity over the sequence range: 229-312.
This sequence record is for human synaptotagmin (p65) (SYT).
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

J C Venter, personal communication

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