Plasmodium falciparum Welch-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

你好,请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
联系电话: 0574-87917803
Plasmodium falciparum Welch
Plasmodium falciparum Welch
规格:
货期:
编号:TS134843
品牌:Testobio
产品名称: Plasmodium falciparum Welch
商品货号: TS134843
Strain Designations: HB-2
Application:
Emerging infectious disease research
Vector borne research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
derived from existing strain
Isolation date: 1981
Product Format: frozen
Storage Conditions: Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Type Strain: no
Comments:
This is a non-gametocyte-forming strain.
Medium: ATCC® Medium 2196: Malaria medium, complete
Growth Conditions:
Temperature: 37°C
Cryopreservation:

Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

  1. Centrifuge ring stage culture for 5 min at 1800rpm in 50 ml centrifuge tube.
  2. Aspirate supernatant using sterile Pasteur pipet.
  3. Resuspend pellet gently in remaining supernatant.
  4. Slowly add 5 volumes of glycerolyte medium (see below) to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  5. Aliquot mixture into Nunc screwtop freezing vials and store at -80°C overnight.
  6. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.

** To formulate glycerolyte medium, combine the following with distilled water to 100 ml:xa0 57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4°C.

Name of Depositor: W Trager
Special Collection: ATCC
Year of Origin: 1981
References:

Bhasin VK, Trager W. Gametocyte-forming and non-gametocyte-forming clones of Plasmodium falciparum. Am. J. Trop. Med. Hyg. 33: 534-537, 1984. PubMed: 6383092

首页 > 产品中心 > 微生物培养 > 菌株 > null > Plasmodium falciparum Welch

Plasmodium falciparum Welch

  • 货号: TS134843
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: Plasmodium falciparum Welch
商品货号: TS134843
Strain Designations: HB-2
Application:
Emerging infectious disease research
Vector borne research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
derived from existing strain
Isolation date: 1981
Product Format: frozen
Storage Conditions: Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Type Strain: no
Comments:
This is a non-gametocyte-forming strain.
Medium: ATCC® Medium 2196: Malaria medium, complete
Growth Conditions:
Temperature: 37°C
Cryopreservation:

Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

  1. Centrifuge ring stage culture for 5 min at 1800rpm in 50 ml centrifuge tube.
  2. Aspirate supernatant using sterile Pasteur pipet.
  3. Resuspend pellet gently in remaining supernatant.
  4. Slowly add 5 volumes of glycerolyte medium (see below) to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  5. Aliquot mixture into Nunc screwtop freezing vials and store at -80°C overnight.
  6. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.

** To formulate glycerolyte medium, combine the following with distilled water to 100 ml:xa0 57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4°C.

Name of Depositor: W Trager
Special Collection: ATCC
Year of Origin: 1981
References:

Bhasin VK, Trager W. Gametocyte-forming and non-gametocyte-forming clones of Plasmodium falciparum. Am. J. Trop. Med. Hyg. 33: 534-537, 1984. PubMed: 6383092

合作单位: