pDP252
pDP252
规格:
货期:
编号:TS135111
品牌:Testobio
| 产品名称： | pDP252 |
|---|---|
| 商品货号： | TS135111 |
| Designations： | pDP252 |
| Species： | Hepatitis B virus, Hepatitis B virus |
| Depositors： | Health Research, Inc., D Panicali, Health Research, Inc. |
| Applications： | This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims. |
| Vector： | Construct size (kb): 5.440000057220459 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: hepatitis B virus Gene symbol: sAg Gene name: surface antigen Contains complete coding sequence?: U Type of DNA: unknown Insert end: HhaI Insert end: HhaI Insert size (kb): 1.08 Cross references: Insert lengths(kb): 1.080000042915344 Gene product: surface antigen sAg |
| Insert Size (kb)： | 1.080 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains a BamHI site. Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 1.15; EcoRI--5.7. The insert contains a BamHI site. This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims. Constructed by cloning a 1.084 kb HhaI fragment of HBV into pBR322 This clone was constructed by isolating a 1.084 kb HhaI fragment from pTHBV containing the coding sequence for the surface antigen without pre-surface antigen. This was blunt-ended with T4 DNA polymerase, linkered with HindIII, and inserted into pBR322. |
| Classification： | Hepadnaviridae, Orthohepadnavirus |
| References： | Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pDP252 |
|---|---|
| 商品货号： | TS135111 |
| Designations： | pDP252 |
| Species： | Hepatitis B virus, Hepatitis B virus |
| Depositors： | Health Research, Inc., D Panicali, Health Research, Inc. |
| Applications： | This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims. |
| Vector： | Construct size (kb): 5.440000057220459 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: hepatitis B virus Gene symbol: sAg Gene name: surface antigen Contains complete coding sequence?: U Type of DNA: unknown Insert end: HhaI Insert end: HhaI Insert size (kb): 1.08 Cross references: Insert lengths(kb): 1.080000042915344 Gene product: surface antigen sAg |
| Insert Size (kb)： | 1.080 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains a BamHI site. Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 1.15; EcoRI--5.7. The insert contains a BamHI site. This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims. Constructed by cloning a 1.084 kb HhaI fragment of HBV into pBR322 This clone was constructed by isolating a 1.084 kb HhaI fragment from pTHBV containing the coding sequence for the surface antigen without pre-surface antigen. This was blunt-ended with T4 DNA polymerase, linkered with HindIII, and inserted into pBR322. |
| Classification： | Hepadnaviridae, Orthohepadnavirus |
| References： | Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
