| 产品名称： | pLW1 |
|---|---|
| 商品货号： | TS135338 |
| Designations： | pLW1 |
| Species： | Homo sapiens, human |
| Depositors： | Cetus Corp., A Belt, Cetus Corp. |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: plasmid Vector end: PstI Vector end: ? Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IL2 Genomic copy number: unique Gene name: interleukin 2 Contains complete coding sequence?: U Chromosome: 4; Localization: 4 q26-q27 Tissue: lymphocyte Type of DNA: cDNA Insert end: HindIII Insert end: PstI Insert size (kb): 0.706 Cross references: Insert lengths(kb): 0.7059999704360962 Tissue: lymphocyte Gene product: interleukin 2 IL2 |
| Insert Size (kb)： | 0.706 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--4.6; EcoRI/PstI--3.7, 0.8; PstI/SstI--4.6. This construct includes the E. coli trp promoter, a ribosome-binding site, and a 706 bp HindIII/PstI fragment encoding IL2. |
| References： | Lin LS, et al. Human recombinant interleukin-2-muteins. US Patent 4,518,584 dated May 21 1985 Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,865,974 dated Sep 12 1989 Gelfand DH, et al. Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element. US Patent 4,666,848 dated May 19 1987 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pLW1 |
|---|---|
| 商品货号： | TS135338 |
| Designations： | pLW1 |
| Species： | Homo sapiens, human |
| Depositors： | Cetus Corp., A Belt, Cetus Corp. |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: plasmid Vector end: PstI Vector end: ? Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IL2 Genomic copy number: unique Gene name: interleukin 2 Contains complete coding sequence?: U Chromosome: 4; Localization: 4 q26-q27 Tissue: lymphocyte Type of DNA: cDNA Insert end: HindIII Insert end: PstI Insert size (kb): 0.706 Cross references: Insert lengths(kb): 0.7059999704360962 Tissue: lymphocyte Gene product: interleukin 2 IL2 |
| Insert Size (kb)： | 0.706 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--4.6; EcoRI/PstI--3.7, 0.8; PstI/SstI--4.6. This construct includes the E. coli trp promoter, a ribosome-binding site, and a 706 bp HindIII/PstI fragment encoding IL2. |
| References： | Lin LS, et al. Human recombinant interleukin-2-muteins. US Patent 4,518,584 dated May 21 1985 Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,865,974 dated Sep 12 1989 Gelfand DH, et al. Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element. US Patent 4,666,848 dated May 19 1987 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
