lambdaSK6 [clone 23]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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联系电话: 0574-87917803
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lambdaSK6 [clone 23]
lambdaSK6 [clone 23]
规格:
货期:
编号:TS135893
品牌:Testobio
产品名称: lambdaSK6 clone 23
商品货号: TS135893
Designations: lambdaSK6 clone 23
Depositors: ER Zabarovsky, G Winberg, G Klein
Biosafety Level: 1
Vector Information:
Size (kb): 43.2000007629394500
Vector: lambdaSK6 (phage, lambda - replacement)
Promoters: Promoter lac
Construction: lambda 2001
Marker(s):red+ gam+
Construct size (kb): 43.20000076293945
Features: insert detection: lacZ, Spi+
marker(s): red+ gam+
promoter: lac
replicon: lambda
Applications:
vector containing primer sites useful for sequencing
vector for constructing genomic libraries
vector for other uses
vector permitting positive selection for inserts
vector permitting visual detection of recombinants
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 13.5, 9.0; EcoRI--20.0, 13.5, 9.0; XhoI--20.0, 13.5, 9.0.
Vector permitting positive selection (Spi) of and visual detection (lacZ) of recombinants. Useful for generating jumping and linking libraries. Also permits construction using partial filling-in.
The polylinker sites attached to each arm are the same.
The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7.
The order of the major features in this plasmid is: arm - XbaI/MCS/AvrII XbaI - lacZO --> - stuffer -
References:

Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275

Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992.

Shipped: frozen
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lambdaSK6 [clone 23]

  • 货号: TS135893
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: lambdaSK6 clone 23
商品货号: TS135893
Designations: lambdaSK6 clone 23
Depositors: ER Zabarovsky, G Winberg, G Klein
Biosafety Level: 1
Vector Information:
Size (kb): 43.2000007629394500
Vector: lambdaSK6 (phage, lambda - replacement)
Promoters: Promoter lac
Construction: lambda 2001
Marker(s):red+ gam+
Construct size (kb): 43.20000076293945
Features: insert detection: lacZ, Spi+
marker(s): red+ gam+
promoter: lac
replicon: lambda
Applications:
vector containing primer sites useful for sequencing
vector for constructing genomic libraries
vector for other uses
vector permitting positive selection for inserts
vector permitting visual detection of recombinants
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 13.5, 9.0; EcoRI--20.0, 13.5, 9.0; XhoI--20.0, 13.5, 9.0.
Vector permitting positive selection (Spi) of and visual detection (lacZ) of recombinants. Useful for generating jumping and linking libraries. Also permits construction using partial filling-in.
The polylinker sites attached to each arm are the same.
The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7.
The order of the major features in this plasmid is: arm - XbaI/MCS/AvrII XbaI - lacZO --> - stuffer -
References:

Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275

Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992.

Shipped: frozen
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